[KD Validated] Anti-MRPL13 Rabbit Monoclonal Antibody (WB, IHC-P, ELISA) - Q9BYD1

Core Product Specifications and Parameters

Biological Background: MRPL13 Function and Localization

• MRPL13 encodes the mitochondrial large ribosomal subunit protein uL13m, also known as 39S ribosomal protein L13, mitochondrial (L13mt, MRP-L13).
• It is a core component of the large subunit (39S) of the mitochondrial ribosome, essential for translating the 13 mitochondria-encoded mRNAs.
• The U13m family is universally conserved across bacteria and eukaryotic organelles, underscoring its fundamental role in ribosome structure and function.
• MRPL13 localizes exclusively to the mitochondrion, where it participates in mitochondrial protein synthesis.
• Post-translational acetylation of MRPL13 has been identified by proteomic studies and may modulate its activity or stability.
• Its 3D structure has been solved as part of the human mitochondrial ribosome, revealing critical interactions with rRNA and other ribosomal proteins.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (aa 70-178) likely accessible in both native and denatured MRPL13, supporting WB, IHC-P, and ELISA applications.
• For Western blot, a specific band around 21 kDa is anticipated; mitochondrial-enriched lysates may improve detection sensitivity.
• In IHC-P, optimization of antigen retrieval (heat- or enzyme-based) is recommended to expose the epitope in formalin-fixed tissues.
• Cross-reactivity with mouse and rat has been observed; validate in the relevant species and sample type for your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide reliable access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-MRPL13 clone. Our offerings cover a broad spectrum of targets for mitochondrial research, from core ribosomal proteins to assembly factors, along with bulk ancillary reagents and custom sourcing. Tell us your project needs, and we’ll bridge the gap from concept to clinic.