[KD Validated] Anti-MRP4/ABCC4 Rabbit Monoclonal Antibody for WB, ELISA - O15439

Core Product Specifications and Parameters

Biological Background: MRP4/ABCC4 Function and Localization

• MRP4/ABCC4 is an ATP-dependent transporter that actively extrudes physiological compounds and xenobiotics from cells. Related references: PMID:11856762, PMID:12523936, PMID:15364914
• Transports cyclic nucleotides (cAMP and cGMP), bile acids, steroid conjugates, urate, and prostaglandins, playing a key role in cellular signaling. Related references: PMID:11856762, PMID:12523936, PMID:12835412
• Mediates the ATP-dependent efflux of glutathione conjugates such as leukotriene C4 (LTC4) and leukotriene B4 (LTB4); GSH is required for LTB4 transport. Related references: PMID:17959747
• Cotransports bile acids with reduced glutathione (GSH). Related references: PMID:12523936, PMID:12883481, PMID:16282361
• Transports a wide range of drugs and metabolites, including anticancer, antiviral, and antibiotic agents, conferring resistance to methotrexate. Related references: PMID:11856762, PMID:12105214, PMID:15454390, PMID:11106685
• Located at both the basolateral and apical cell membranes, with widespread tissue expression; highest in prostate but barely detectable in liver sinusoidal membrane.
• Belongs to the ATP-binding cassette (ABC) transporter family, featuring two ATP-binding domains, multiple transmembrane helices, and subject to alternative splicing and post-translational modifications (phosphorylation, glycosylation).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 1067-1325) of human MRP4/ABCC4, ensuring recognition of the full-length transporter.
• For Western blot, expect a band near 150 kDa; use appropriate molecular weight markers and consider optimized membrane protein extraction protocols.
• This antibody is KD validated, supporting its use in specificity verification via gene silencing experiments.
• In ELISA applications, consider using cell lysates or purified protein, and validate the assay with appropriate controls in the relevant sample system.
• Cross-reactivity with mouse and rat allows applications in rodent model studies; however, verify performance individually in each species.

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