[KD Validated] Anti-MIF Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P14174

Core Product Specifications and Parameters

Biological Background: MIF Function and Localization

• MIF acts as a pro-inflammatory cytokine, playing a critical role in the innate immune response to bacterial pathogens, regulating macrophage function in host defense, and counteracting the anti-inflammatory effects of glucocorticoids. Related references: PMID:15908412, PMID:17443469, PMID:23776208
• In vitro, MIF exhibits phenylpyruvate tautomerase and dopachrome tautomerase activity, though the physiological relevance of these enzymatic activities remains unclear. Related references: PMID:11439086, PMID:17526494
• Also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, and phenylpyruvate tautomerase, reflecting its multifunctional nature.
• It localizes to both the cytoplasm and is secreted, consistent with its role as an extracellular cytokine.
• Classified under keywords including cytokine, inflammatory response, innate immunity, and isomerase, highlighting its dual roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 2–115 of human MIF, a region highly conserved across species; cross-reactivity with mouse and rat is indicated.
• For Western blot, the predicted molecular weight is 12 kDa; consider using high-percentage gels and optimized transfer conditions for small proteins.
• In immunofluorescence/ICC, validate fixation and permeabilization protocols to detect both cytoplasmic and secreted MIF pools.
• For ELISA, perform appropriate optimization and include relevant positive and negative controls to ensure assay specificity.

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CamelBio provides diagnostic manufacturers and researchers with a streamlined, one-stop channel for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert technical consulting. Our portfolio supports inflammation and immunity research, with polyclonal antibodies like this anti-MIF product enabling reliable detection across multiple applications. Partner with CamelBio to accelerate your assay development from concept to clinic with confidence.