[KD Validated] Anti-METTL3 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - Q86U44

Core Product Specifications and Parameters

Biological Background: METTL3 Function and Localization

• METTL3 is the catalytic subunit of the METTL3-METTL14 heterodimeric N6-adenosine-methyltransferase complex that installs m6A modifications on RNA (PubMed:22575960, PubMed:24284625, PubMed:25719671, PubMed:27281194, PubMed:27373337, PubMed:27627798). Related references: PMID:22575960, PMID:24284625, PMID:25719671, PMID:27281194, PMID:27373337, PMID:27627798
• m6A methylation promotes mRNA destabilization and degradation, serving as a key regulator of mRNA turnover (PubMed:28637692). Related references: PMID:28637692
• METTL3-mediated m6A methylation participates in DNA damage response by rapidly modifying poly(A) transcripts at UV-induced damage sites, recruiting POLK (PubMed:28297716). Related references: PMID:28297716
• METTL3 contributes to the regulation of the circadian clock and circadian hepatic lipid metabolism (PubMed:30428350). Related references: PMID:30428350
• METTL3 methylates pri-miRNAs, marking them for DGCR8 recognition and processing (PubMed:25799998). Related references: PMID:25799998
• METTL3 catalyzes m6A on Xist RNA, promoting Xist-mediated transcriptional repression during random X inactivation (PubMed:27602518). Related references: PMID:27602518
• METTL3 can promote mRNA translation independently of its methyltransferase activity by interacting with translation initiation factors in the cytoplasm; its overexpression is linked to cancer cell proliferation (PubMed:27117702). Related references: PMID:27117702
• During SARS-CoV-2 infection, METTL3 adds m6A modifications to viral RNA, dampening RIG-I binding and suppressing innate immune responses (PubMed:33961823). Related references: PMID:33961823
• Subcellular localization is primarily nuclear, enriched in nuclear speckles, and also detected in the cytoplasm. It is widely expressed at low levels, notably in spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood leukocytes. It is a phosphoprotein, acetylated, and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• This antibody was raised against amino acids 2-250 of human METTL3, corresponding to the N-terminal region containing the MT-A70 domain. It is expected to detect full-length METTL3 at ~64 kDa in WB.
• For IF/ICC, anticipate nuclear and nuclear speckle staining patterns; consider co-staining with nuclear markers.
• In IP experiments, confirm specificity by including negative control IgG and validate co-immunoprecipitation of METTL14 if studying the complex.
• Cross-reactivity with mouse and rat makes it suitable for rodent model studies; however, validate in the relevant tissue or cell line.
• Optimize antibody dilution for each application; typical starting dilutions are not provided, so empirical titration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we streamline your journey from research to clinic by providing validated IVD raw materials, including this high-quality METTL3 monoclonal antibody for epitranscriptomics and RNA modification studies. Our portfolio extends to optimized antibody pairs, bulk ancillary reagents, and rare target sourcing, ensuring seamless integration into your diagnostic development workflows. Trust CamelBio as your reliable partner for comprehensive, one-stop raw-material solutions tailored to advance gene regulation and disease research.