KD Validated Anti-ME1 Rabbit Polyclonal Antibody for WB and ELISA - P48163

Core Product Specifications and Parameters

Biological Background: ME1 Function and Localization

• ME1, also known as NADP-dependent malic enzyme or malic enzyme 1, is encoded by the ME1 gene in humans.
• It catalyzes the oxidative decarboxylation of (S)-malate to pyruvate in the presence of NADP+ and divalent metal ions, producing NADPH.
• Additionally, ME1 can catalyze the decarboxylation of oxaloacetate, a key anaplerotic reaction.
• As a member of the malic enzyme family, ME1 is a cytosolic enzyme and is listed under the oxidoreductase family.
• The protein is ubiquitously expressed, with notable expression in liver, placenta, and white adipose tissue.
• ME1 is post-translationally modified by acetylation and phosphorylation, influencing its activity and regulation.
• Alternative splicing of the ME1 gene results in multiple transcript variants.
• The crystal structure of ME1 has been determined, providing insights into its catalytic mechanism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 472-572) of human ME1, which may recognize epitopes conserved across species, consistent with the reported cross-reactivity with mouse and rat.
• For Western blotting (WB), a band around 64 kDa is expected; consider using appropriate positive controls (e.g., human liver or adipose tissue lysates) to verify specificity.
• ELISA performance may depend on coating conditions and detection substrates; optimization using recombinant ME1 protein as standard could enhance assay sensitivity.
• Given the ubiquitous expression, validate antibody performance in the specific tissue or cell line of interest to ensure detection of ME1 in the relevant biological context.
• Since this is a polyclonal antibody, lot-to-lot consistency should be confirmed via titration experiments.

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