[KD Validated] Anti-MAD1/MAD1L1 Polyclonal Antibody for WB, ELISA - Q9Y6D9

Core Product Specifications and Parameters

Biological Background: MAD1/MAD1L1 Function and Localization

• MAD1L1 (also known as MAD1, TXBP181) encodes the mitotic spindle assembly checkpoint protein MAD1, a critical guardian of mitotic fidelity.
• It forms a heterotetrameric complex with closed MAD2L1 (C-MAD2) at unattached kinetochores, promoting conversion of open MAD2L1 (O-MAD2) to C-MAD2 to propagate the “wait anaphase” signal. Related references: PMID:10049595 PMID:20133940 PMID:29162720
• In hepatocellular carcinoma, MAD1 can sequester MAD2L1 in the cytoplasm, impairing the spindle assembly checkpoint and contributing to chromosomal instability.
• The protein dynamically localizes to kinetochores, centrosomes, spindle poles, and the nuclear envelope during mitosis, and also shuttles through the cytoplasm.
• MAD1 is subject to post-translational modifications, including phosphorylation and acetylation, which regulate its cell-cycle-dependent functions.
• Tissue expression is notable in hepatocellular carcinomas and hepatoma cell lines, aligning with its oncogenic relevance.
• Alternative splicing of MAD1L1 generates multiple isoforms, and mutations are associated with cancer-related checkpoint defects.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 351–718 of human MAD1, covering a large C-terminal region conserved across isoforms; this polyclonal antibody may therefore recognize multiple variants.
• For Western blot, the expected molecular weight is approximately 83 kDa; use a 10% SDS-PAGE gel and ensure complete transfer for high-molecular-weight proteins.
• ELISA reactivity should be validated under the specific assay conditions; consider checkerboard titrations to determine optimal coating antigen and antibody concentrations.
• As with any polyclonal reagent, lot-to-lot consistency should be confirmed using a standardized cell lysate or recombinant protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, technical services, and consultative expertise—from concept to clinic. For mitosis and oncology-focused projects, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing capabilities for rare targets like MAD1/MAD1L1. Partner with us to streamline your assay development and secure critical raw materials efficiently.