[KD Validated] Anti-LYPLA1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O75608

Core Product Specifications and Parameters

Biological Background: LYPLA1 Function and Localization

• Acyl-protein thioesterase 1 (LYPLA1/APT1), also known as lysophospholipase 1 (LPL1), is a ~25 kDa hydrolase of the AB hydrolase superfamily.
• Acts as a potent palmitoyl thioesterase, mediating the depalmitoylation of diverse substrates including G alpha proteins, HRAS, ADRB2, KCNMA1, and SQSTM1. Related references: PMID:20418879, PMID:22399288, PMID:27481942, PMID:37802024
• Functions as a lysophospholipase, hydrolyzing lysophosphatidylcholine and other lysophospholipids, but thioesterase activity predominates. Related references: PMID:19439193
• Contributes to extracellular lysophosphatidic acid (LPA) production during coagulation by supplying substrate lysophospholipids to autotaxin (ENPP2). Related references: PMID:21393252
• Serves as a negative regulator of autophagy through SQSTM1 depalmitoylation, which disrupts its interaction with ATG8 family proteins. Related references: PMID:37802024
• Primarily localized to the cytoplasm, and also detected at the cell membrane, nuclear membrane, and endoplasmic reticulum.
• Expressed in platelets, with roles in lipid metabolism and protein palmitoylation dynamics.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is 25 kDa; consider using appropriate gel percentage and blocking conditions.
• For IHC-P, antigen retrieval methods may be required; validate in the relevant tissue (e.g., platelets or tissues expressing LYPLA1).
• For ELISA, use as capture or detection antibody after pairing validation.
• Since the immunogen covers the N-terminal 230 amino acids of human LYPLA1, cross-reactivity with mouse and rat is expected due to high sequence homology (verify by sequence alignment).
• The KD validation indicates this antibody can detect endogenous LYPLA1; consider siRNA or knockout controls to confirm specificity.

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