[KD Validated] Anti-LXR beta/NER Rabbit Monoclonal Antibody for WB, IP, ELISA - P55055

Core Product Specifications and Parameters

Biological Background: LXR beta/NER Function and Localization

• LXR beta (NR1H2) is a member of the nuclear receptor superfamily and serves as an oxysterol receptor, exhibiting ligand-dependent transcriptional activation. Related references: PMID:25661920
• It binds to specific DNA sequences known as direct repeat 4 (DR-4) elements, characterized by the consensus half-site AGGTCA separated by 4 nucleotides.
• LXR beta plays a key role in cholesterol metabolism by promoting MYLIP-mediated ubiquitination and subsequent degradation of LDLR, VLDLR, and LRP8 receptors.
• In hepatocytes, it induces LPCAT3-dependent phospholipid remodeling in the ER membrane, which drives SREBF1 processing, lipogenesis, and VLDL particle formation.
• The receptor also counteracts lipid-induced ER stress and inflammation, likely by modulating SRC kinase membrane compartmentalization.
• During the hepatic acute phase response, LXR beta acts as a transcriptional corepressor by preventing the dissociation of the N-Cor corepressor complex, exerting an anti-inflammatory effect. Related references: PMID:20159957
• LXR beta is predominantly nuclear, consistent with its function as a transcription factor.
• It is ubiquitously expressed and contains zinc-finger motifs, indicative of direct DNA binding and regulation.

Experimental Guidance and Technical Tips

• This monoclonal antibody was generated against an N-terminal peptide (aa 1-100) of human LXR beta. For Western blotting, consider using reduced/denatured conditions and validate the expected band size of approximately 51 kDa in relevant human cell lysates.
• For immunoprecipitation, optimize the antibody-to-lysate ratio and include appropriate IgG controls.
• ELISA applications may require optimization of coating antigen and detection conditions based on your specific assay format.
• Cross-reactivity with other species has not been tested; if using non-human samples, validate specificity and sensitivity independently.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we support IVD manufacturers and research laboratories with reliable access to raw materials like anti-LXR beta antibodies, along with ancillary reagents for lipid metabolism and nuclear receptor research. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies, and bulk supply options to streamline your assay development from concept to clinic. Contact us for rare-target sourcing and custom technical consulting services.