[KD Validated] Anti-LC3B Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9GZQ8

Core Product Specifications and Parameters

Biological Background: LC3B Function and Localization

• LC3B (MAP1LC3B) is a ubiquitin-like modifier essential for the formation of autophagosomal vacuoles during macroautophagy.
  Related references:
  PMID:20418806, PMID:23209295, PMID:28017329

• It plays a critical role in mitophagy by binding C-18 ceramides and anchoring autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria.
  Related references:
  PMID:22922758

• LC3B promotes primary ciliogenesis through autophagy-dependent removal of OFD1 from centriolar satellites.
  Related references:
  PMID:24089205

• Upon nutrient stress, LC3B interacts with reticulophagy receptor TEX264 to remodel endoplasmic reticulum subdomains into autophagosomes for ER turnover.
  Related references:
  PMID:31006537, PMID:31006538

• LC3B directly recruits JMY to the phagophore membrane, stimulating actin nucleation and autophagosome biogenesis.
  Related references:
  PMID:30420355

• LC3s are primarily involved in phagophore elongation, whereas the GABARAP/GATE-16 subfamily functions at a later stage of autophagosome maturation.
  Related references:
  PMID:20418806, PMID:23209295, PMID:28017329

• Subcellularly, LC3B localizes to autophagosome membranes, mitochondrial membranes, cytoplasmic vesicles, and the cytoskeleton.

• Tissue expression: most abundant in heart, brain, skeletal muscle, and testis; low in liver.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100) of human LC3B, which is present in both the unconjugated (LC3B-I) and lipidated (LC3B-II) forms. Consider this design when interpreting results across different autophagic states.
• For Western blotting, a starting dilution of 1:500–1:2000 is often suitable; however, optimization in your experimental system is recommended. Expect to observe two bands corresponding to LC3B-I and LC3B-II, with LC3B-II migrating slightly faster.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), include appropriate controls (e.g., autophagy induction) and validate signal specificity with knockdown or knockout samples if available.
• Since the antibody shows cross-reactivity with mouse and rat LC3B, confirm sequence homology if using these species, and consider performing a pilot study to assess performance in non-human samples.

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