[KD Validated] Anti-ISG15 Rabbit Monoclonal Antibody for WB, ELISA - P05161

Core Product Specifications and Parameters

Biological Background: ISG15 Function and Localization

• ISG15 (interferon-stimulated gene 15) is a 15-17 kDa ubiquitin-like protein, also known as G1P2, IP17, and hUCRP, encoded by the ISG15 gene on human chromosome 1.
• It plays a critical role in innate antiviral immunity by conjugating to lysine residues of target proteins (ISGylation), a process involving E1, E2, and E3 enzymes. Related references: PMID:27564865 PMID:39465252 PMID:33727702
• ISGylation promotes oligomerization of the viral sensor IFIH1/MDA5, activating innate immunity against coronaviruses, flaviviruses, and picornaviruses. Related references: PMID:33727702
• Free, unconjugated ISG15 is secreted and acts as a cytokine, inducing IFN-γ production and NK cell proliferation, and serving as a chemotactic factor for neutrophils. Related references: PMID:29100055
• Subcellularly, ISG15 is found in the cytoplasm and can be secreted into the extracellular space.
• It exhibits broad antiviral activity against both DNA and RNA viruses, including influenza A, HIV-1, and Ebola virus, by disrupting viral budding and replication.
• ISG15 is expressed in lymphoid cells, striated and smooth muscle, various epithelia, and neurons. Its expression is enhanced in several cancers, including pancreatic adenocarcinoma and bladder cancer.
• Post-translational modifications include S-nitrosylation and disulfide bond formation, and it participates in the ubiquitin-like conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human ISG15 (amino acids 1-165), suggesting antibody recognition of the native protein. Confirm specificity in your sample matrix.
• For western blot, the expected band is at ~18 kDa under reducing conditions. Use appropriate positive controls (e.g., interferon-treated cells) to verify detection.
• Cross-reactivity with mouse and rat ISG15 is reported; however, validate reactivity in your specific experimental system, as species homology may affect signal intensity.
• ELISA protocols may require optimization of coating antigen and antibody concentration. Consider using recombinant ISG15 protein as a standard.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide diagnostic manufacturers and research institutions with seamless access to high-quality IVD raw materials, including validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents. This ISG15 monoclonal antibody is ideal for developing assays targeting innate immunity and infectious disease biomarkers. For rare targets or custom sourcing requests, our dedicated team offers one-stop support to bridge your concepts to clinical applications.