[KD Validated] Anti-ISG15 Polyclonal Antibody for WB, ELISA - P05161

Core Product Specifications and Parameters

Biological Background: ISG15 Function and Localization

• ISG15 (Interferon-stimulated gene 15) is a ubiquitin-like protein also known as G1P2, IP17, or hUCRP, encoded by the ISG15 gene.
• It plays a key role in innate immunity, acting either via conjugation to target proteins (ISGylation) or as a free secreted cytokine.
  Related references: PMID:27564865 PMID:39465252
• ISGylation involves E1, E2, and E3 enzymes and targets proteins such as IFIT1, MX1, and the viral sensor IFIH1/MDA5, promoting antiviral signaling.
  Related references: PMID:33727702
• Exhibits broad antiviral activity against DNA and RNA viruses, including influenza A, HIV-1, and Ebola, by disrupting viral budding or protein function.
• The secreted form of ISG15 acts as a cytokine, inducing natural killer cell proliferation, neutrophil chemotaxis, and IFN-gamma production, essential for antimycobacterial immunity.
  Related references: PMID:29100055
• ISG15 is localized in the cytoplasm and also secreted; expression is found in lymphoid cells, muscle, epithelia, and neurons, and is upregulated in certain cancers.
• Post-translational modifications include disulfide bond formation and S-nitrosylation; ISG15 is part of the ubiquitin-like conjugation pathway.

Experimental Guidance and Technical Tips

• This antibody was generated against a recombinant protein covering amino acids 1–165 of human ISG15, corresponding to the full-length canonical isoform; it is expected to detect the ~18 kDa protein in Western blotting.
• For WB, consider using standard lysates from interferon-stimulated human cells to confirm ISG15 upregulation; a single band near 18 kDa should be observed.
• Cross‑reactivity is reported for human samples only; if extending to other species, sequence homology should be checked and validation performed.
• As a polyclonal antibody, lot‑to‑lot consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti‑ISG15 antibody supports research into antiviral innate immunity and ISGylation pathways. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary supplies, and rare‑target raw‑material sourcing to streamline your diagnostic development.