[KD Validated] Anti-Integrin alpha 5 (ITGA5/CD49e) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P08648

Core Product Specifications and Parameters

Biological Background: Integrin alpha-5 Function and Localization

• Integrin alpha-5 (ITGA5), also known as CD49e, VLA-5 alpha chain, or fibronectin receptor subunit alpha, pairs with integrin beta-1 (ITGB1) to form the major fibronectin receptor.
• ITGA5:ITGB1 binds fibronectin and fibrinogen, recognizing the R-G-D tripeptide motif, and mediates cell adhesion and signaling. Related references: PMID:33962943
• The integrin also acts as a receptor for fibrillin-1 (FBN1), contributing to R-G-D-dependent cell adhesion to extracellular matrix components. Related references: PMID:12807887, PMID:17158881
• ITGA5:ITGB1 serves as a receptor for interleukin-1 beta (IL1B), essential for IL1B signaling, and in the context of ITGA5:ITGB3, it functions as a receptor for soluble CD40LG, participating in CD40/CD40LG signaling. Related references: PMID:29030430, PMID:31331973
• Additionally, ITGA5:ITGB1 binds PLA2G2A at a distinct site, inducing conformational changes that enhance ligand binding to the classical site. Related references: PMID:18635536, PMID:25398877
• The protein is localized at the cell membrane and focal adhesions, underscoring its role in cell-matrix interactions.
• ITGA5 is expressed in placenta and is involved in various host-virus interactions, including acting as a receptor for Human metapneumovirus and mediating HIV-1 Tat protein-induced angiogenesis.
• Post-translational modifications include phosphorylation, disulfide bond formation, and glycosylation, typical of integrin family members.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 42-995 of human ITGA5, covering the majority of the extracellular domain, which may contribute to recognition of the native protein.
• For Western blot, the predicted molecular weight is 115 kDa; validate the band size in your specific sample system.
• IF/ICC experiments may require optimization of fixation and permeabilization protocols, given ITGA5's membrane and focal adhesion localization.
• ELISA performance should be validated against the relevant target protein and sample matrix.
• Consider that cross-reactivity is reported only for human; reactivity with other species should be verified independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, providing diagnostic manufacturers and research labs with validated antibodies, bulk reagents, and tailored sourcing solutions. This anti-ITGA5 monoclonal antibody supports cell adhesion and integrin signaling research, and we offer comprehensive procurement services for related targets. From antibody pairs to rare target raw materials, CamelBio streamlines your supply chain from concept to clinic.