KD Validated Anti-IL13 Monoclonal Antibody for WB and ELISA - P35225

Core Product Specifications and Parameters

Biological Background: IL13 Function and Localization

• Interleukin-13 (IL-13, also known as P600) is a key cytokine in allergic inflammation, asthma, and immune responses against parasitic infections (PubMed:8096327, PubMed:8097324). Related references: PMID:8096327 PMID:8097324
• Synergizes with IL2 to regulate interferon-gamma synthesis, modulating Th1/Th2 balance (PubMed:8096327). Related references: PMID:8096327
• Promotes B-cell proliferation and activates eosinophils, basophils, and mast cells, contributing to allergic effector mechanisms (PubMed:7903680, PubMed:8759755). Related references: PMID:7903680 PMID:8759755
• Downregulates proinflammatory cytokines (IL1, IL6, IL10, IL12, TNF) partly via NF-kappa-B suppression, and modulates IL33 activity by influencing IL1RL1 expression (By similarity).
• Acts on endothelial cells to induce VCAM1, facilitating eosinophil recruitment during allergic responses (PubMed:8639787). Related references: PMID:8639787
• Signals through IL4R/IL13RA1, activating JAK1/TYK2 and STAT6; IL13RA2 serves as a high-affinity decoy receptor, depleting extracellular IL13 (PubMed:9013879, PubMed:21622864). Related references: PMID:9013879 PMID:21622864
• Induces esophageal epithelial inflammation and disrupts desmosomal junctions by downregulating DSG1, relevant to eosinophilic esophagitis (PubMed:24220297). Related references: PMID:24220297
• Secreted glycoprotein with disulfide bonds; molecular weight approximately 16 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 25–146 of human IL13, covering the mature secreted form; this design likely provides recognition of native IL13.
• For Western blot (WB), consider testing at an initial antibody dilution of 1:500–1:5000 depending on sample abundance, and optimize loading controls.
• ELISA applications may require pairing with a detection antibody recognizing a non-overlapping epitope; validate in the relevant sample system (e.g., cell culture supernatant or serum).
• Confirm cross-reactivity and specificity in the intended assay system, as stated reactivities are limited to human IL13.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, technical services, and consulting for diagnostic manufacturers and laboratories. For IL13-related immunoassay development, we supply validated monoclonal antibodies, antigen-grade proteins, and ancillary reagents. Whether you need bulk quantities, customized formulations, or support from concept to clinic, CamelBio is your trusted sourcing partner.