KD Validated Anti-HNRNPA2B1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P22626

Core Product Specifications and Parameters

Biological Background: HNRNPA2B1 Function and Localization

• Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2/B1) is a multifunctional RNA-binding protein that packages nascent pre-mRNAs into hnRNP particles, ensuring transcript stability and preventing tangling. Related references: PMID:19099192
• It specifically recognizes the A2RE sequence motif on certain mRNAs (e.g., in oligodendrocytes and neurons) and mediates their cytoplasmic transport, a critical process for local protein synthesis. Related references: PMID:10567417
• As a nuclear reader of N6-methyladenosine (m6A) modifications, hnRNPA2/B1 binds m6A-marked primary miRNAs and pre-mRNAs, promoting pri-miRNA processing by DGCR8 and modulating splicing efficiency. Related references: PMID:26321680, PMID:24356509
• The protein also regulates alternative splicing; for example, it represses exon 9 inclusion in pyruvate kinase PKM pre-mRNA, favoring the M2 isoform production with implications in cancer metabolism. Related references: PMID:20010808
• In innate immunity, upon sensing nuclear viral DNA, hnRNPA2/B1 homodimerizes, is demethylated by JMJD6, and translocates to the cytoplasm where it activates the TBK1-IRF3 pathway, triggering interferon production. Related references: PMID:31320558
• Subcellularly, hnRNPA2/B1 is predominantly nuclear but shuttles to the cytoplasm, and it is also found in secreted exosomes and cytoplasmic granules; post-translational modifications include acetylation, methylation, phosphorylation, and sumoylation.

Experimental Guidance and Technical Tips

• For WB, the predicted molecular weight is ~37 kDa; consider using appropriate loading controls and validate in human, mouse, or rat lysates.
• For IHC-P, antigen retrieval methods may be required; optimize conditions on positive control tissues known to express hnRNPA2/B1.
• ELISA: Determine optimal antibody dilution and coating antigen concentration through pilot experiments.
• This polyclonal antibody was raised against an internal peptide (aa 150-250); verify specificity in your assay system, particularly when distinguishing isoforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this KD validated anti-HNRNPA2B1 rabbit polyclonal antibody as part of a comprehensive portfolio for RNA biology and innate immunity research. As a one-stop IVD raw material supplier, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare targets to accelerate diagnostic development from concept to clinic.