[KD Validated] Anti-GPX4 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P36969

Core Product Specifications and Parameters

Biological Background: GPX4 Function and Localization

• GPX4, also known as phospholipid hydroperoxide glutathione peroxidase (PHGPx), directly reduces phospholipid hydroperoxides within membranes and lipoproteins, serving as a crucial antioxidant enzyme (PubMed:40281343). Related references: PMID:40281343
• It plays an essential role in inhibiting ferroptosis, an iron-dependent non-apoptotic cell death triggered by lipid peroxidation (PubMed:24439385, PubMed:40281343). Related references: PMID:24439385, PMID:40281343
• The enzyme also reduces cholesterol hydroperoxide, thymine hydroperoxide, fatty acid-derived hydroperoxides (PubMed:11115402, PubMed:36608588), and small soluble hydroperoxides such as H2O2, cumene hydroperoxide, and tert-butyl hydroperoxide (PubMed:17630701, PubMed:36608588). Related references: PMID:11115402, PMID:36608588, PMID:17630701
• GPX4 localizes to both mitochondria and cytoplasm, enabling protection of organelle and cellular membranes against oxidative stress.
• Tissue expression is highest in testis, and the protein is also detected in platelets, where it participates in arachidonic acid metabolism (PubMed:11115402). Related references: PMID:11115402
• The active site selenocysteine (Sec) residue is essential for catalytic activity and provides resistance to overoxidation, a key factor in ferroptosis prevention.
• GPX4 is required for normal sperm development, male fertility, and photoreceptor cell maturation and survival.
• Post-translational modifications include alternative initiation, hydroxylation, and phosphorylation; the protein is classified under lipid metabolism and oxidative stress pathways.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 74-197 of human GPX4, a region expected to be conserved and accessible in the native protein.
• For Western blot, a band at approximately 22kDa is anticipated; reducing SDS-PAGE conditions are recommended.
• In IHC-P, antigen retrieval may enhance detection; validation on known GPX4-expressing tissues such as testis is advised.
• For IF/ICC, optimal fixation and permeabilization protocols should be determined empirically for the cell type of interest.
• Cross-reactivity with mouse and rat GPX4 suggests suitability for rodent models; however, confirm sequence homology in the immunogen region if precise species reactivity is critical.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to streamline IVD raw material sourcing, from antibody development to bulk supply. This anti-GPX4 polyclonal antibody supports studies in ferroptosis, oxidative stress, and lipid metabolism, and complements our catalog of validated antibody pairs, monoclonal/polyclonal reagents, and ancillary biochemicals. For customized or high-volume requirements, our team offers tailored procurement solutions, ensuring seamless integration into your assay development pipeline.