[KD Validated] Anti-GPLD1 Rabbit Polyclonal Antibody for WB, ELISA - P80108

Core Product Specifications and Parameters

Biological Background: GPLD1 Function and Localization

• GPLD1 (Phosphatidylinositol-glycan-specific phospholipase D), also known as Glycoprotein phospholipase D, GPI-PLD, or GPI-specific phospholipase D, is an enzyme that hydrolyzes the inositol phosphate linkage of GPI-anchored membrane proteins, releasing them from the cell surface. This cleavage occurs on the inositol side of the anchor, leaving the phosphate group attached to the membrane, and modulates diverse biological processes including signaling pathway activation. Related references: PMID:17720976 PMID:2760042
• The protein is secreted and functions extracellularly, consistent with its role in shedding GPI-anchored proteins from the plasma membrane.
• GPLD1 is a glycoprotein and belongs to the phospholipase D family, featuring repeat sequences and playing a key role in lipid metabolism.
• Alternative splicing of the GPLD1 gene produces multiple isoforms, which may have distinct tissue distributions or activities.
• The predicted molecular weight is approximately 92 kDa (UniProt: 92,336 Da), aligning with the observed band in Western blot.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 24–160) of human GPLD1, potentially detecting multiple isoforms or cleavage products; confirm band specificity with appropriate controls.
• For Western blot, expect a dominant band near 92 kDa in human or mouse samples (e.g., serum, plasma, or cell lysates); optimize loading amounts and antibody dilutions for your system.
• In ELISA, use recombinant GPLD1 or conditioned medium from cells expressing GPLD1 as a standard; validate reactivity against mouse GPLD1 if cross-species detection is required.
• Consider pre-adsorption or blocking steps if non-specific binding is observed, and always include a negative control (e.g., GPLD1 knockout lysate) when available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a single access point for IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents. This anti-GPLD1 antibody supports research into GPI anchor biology, cell signaling, and lipid metabolism. For scalable sourcing, rare target material procurement, or end-to-end development support, contact CamelBio to bridge your concept to clinic.