[KD Validated] Anti-glucose-6-phosphate isomerase Rabbit mAb for WB, ELISA - P06744

Core Product Specifications and Parameters

Biological Background: Glucose-6-phosphate isomerase Function and Localization

• Catalyzes the conversion of alpha-D-glucose-6-phosphate to beta-D-fructose-6-phosphate, the second step in glycolysis, and the reverse reaction in gluconeogenesis. Related references: PMID:28803808
• Also displays C2-epimerase and anomerase activities, interconverting D-glucose-6-phosphate and D-mannose-6-phosphate, and alpha/beta anomeric forms.
• Functions as autocrine motility factor (AMF), a secreted angiogenic factor that enhances endothelial cell motility. Related references: PMID:11437381
• Acts as neuroleukin (NLK), a neurotrophic factor supporting the survival of spinal and sensory neurons. Related references: PMID:11004567 PMID:3352745
• Released by lectin-stimulated T-cells to induce immunoglobulin secretion. Related references: PMID:11004567 PMID:3352745
• Subcellular localization: predominantly cytoplasmic, but also secreted.
• Post-translational modifications include acetylation, hydroxylation, phosphorylation, and ubiquitination.
• Associated with hereditary hemolytic anemia when mutated (keywords: disease variant).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 of human GPI; consider validating reactivity in your human sample system before large-scale experiments.
• For WB, use whole-cell lysates from human cell lines and expect a band near 63 kDa; validate the knockdown efficiency with appropriate controls.
• For ELISA, optimize antigen coating and antibody dilution to ensure specificity; consider paired detection reagents for quantitative assays.
• Since the antibody is KD validated, confirm gene silencing in your experimental setup and include scrambled shRNA/siRNA controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a streamlined one-stop channel for IVD raw materials, technical services, and consulting from concept to clinic. Beyond this anti-GPI antibody, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your glycolysis, cancer metabolism, and cytokine signaling research.