[KD Validated] Anti-GALC Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P54803

Core Product Specifications and Parameters

Biological Background: GALC Function and Localization

• GALC hydrolyzes the galactose ester bonds of glycolipids including galactosylceramide and galactosylsphingosine, and mediates lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney, and epithelia. Related references: PMID:8281145, PMID:8399327
• The protein belongs to the glycosyl hydrolase 59 family and is highly conserved among human, mouse, and rat.
• Alternative splicing of GALC transcripts produces multiple isoforms, which may have distinct regulatory or functional roles.
• Subcellularly, GALC localizes to the lysosome, consistent with its acid hydrolase activity.
• GALC is expressed in various tissues, with detectable levels in testis, brain, placenta, urine, kidney, and liver.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are critical for enzyme folding and stability.
• Loss-of-function mutations in GALC lead to Krabbe disease (globoid cell leukodystrophy), characterized by severe demyelination and neurodegeneration.

Experimental Guidance and Technical Tips

• For Western blot, a starting dilution of 1:500–1:2,000 is often suitable for detecting the ~77 kDa GALC band in cell or tissue lysates. Validate in relevant samples such as brain, testis, or kidney.
• In immunohistochemistry (paraffin), antigen retrieval using citrate buffer (pH 6.0) may improve staining. Consider using positive control tissues like brain or placenta.
• ELISA applications can employ the antibody as a capture or detection reagent; optimal pairing and concentrations should be determined using recombinant GALC or calibrated biological samples.
• The antibody recognizes human, mouse, and rat GALC, making it useful for cross-species comparative studies.
• As a knockdown-validated mAb, it may provide reliable target specificity in Western blot assays; users should confirm knockdown efficiency in their own experimental system.
• Storage and handling: aliquot and store at -20°C for long-term stability; avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD manufacturers, offering this high-quality anti-GALC monoclonal antibody alongside a comprehensive portfolio of validated antibody pairs, optimized monoclonals, and bulk ancillary reagents. We specialize in bridging rare and critical raw materials for diagnostic development, supporting research into lysosomal storage disorders and neurodegenerative diseases. Contact our team to streamline your supply chain from concept to clinic.