[KD Validated] Anti-Ferritin Light Chain (FTL) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P02792

Core Product Specifications and Parameters

Biological Background: Ferritin Light Chain Function and Localization

• Human ferritin light chain (FTL) is a ~20 kDa iron-storage protein belonging to the ferritin family, crucial for cellular iron homeostasis.
• Stores iron in a soluble, non-toxic form by taking up ferrous iron and depositing it as ferric hydroxides after oxidation, thus preventing oxidative stress.
• Mediates iron delivery to cells; for example, facilitates iron uptake in capsule cells of the developing kidney (by similarity).
• Delivered to lysosomes by the cargo receptor NCOA4 for autophagic degradation and subsequent release of iron. Related references: PMID:24695223
• Localizes predominantly in the cytoplasm but also resides in cytoplasmic vesicles, autophagosomes, and autolysosomes.
• Post-translational modifications include acetylation, as identified by proteomics approaches.
• Mutations in the FTL gene are associated with neurodegeneration with brain iron accumulation type 3 (NBIA3) and hereditary hyperferritinemia-cataract syndrome.
• Keywords highlight its roles in iron storage, metal binding, and disease variants involving cataract and neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human ferritin light chain (aa 1-175), suggesting recognition of the native protein in properly folded conformation.
• For WB, a single band at ~20 kDa is expected under reducing conditions; consider using positive controls such as HeLa cell or liver lysates.
• In IHC-P, antigen retrieval methods may need optimization; validate staining specificity using known positive and negative tissue sections.
• Cross-reactivity with mouse and rat allows translational studies in rodent models, but verify sequence homology before use.
• Optimal antibody dilutions and incubation times should be determined empirically for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For projects focusing on iron metabolism, neurodegeneration, or ferritin biology, we streamline supply of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials. Reach out to our team for customized sourcing solutions and reliable batch-to-batch consistency.