[KD Validated] Anti-ERK1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P27361

Core Product Specifications and Parameters

Biological Background: ERK1 Function and Localization

• ERK1 (extracellular signal-regulated kinase 1), encoded by the MAPK3 gene, is a serine/threonine kinase also known as p44-MAPK or microtubule-associated protein 2 kinase.
• It serves as an essential component of the MAPK/ERK signal transduction cascade, mediating cell growth, adhesion, survival, and differentiation (PubMed:34497368).
  Related references: PMID:34497368
• Along with ERK2 (MAPK1), ERK1 is one of the two primary MAPKs that propagate signals from activated receptors like KIT to downstream targets.
• ERK1 phosphorylates over 160 substrates, including transcription factors (e.g., ATF2, ELK1), cytoskeletal proteins, apoptosis regulators, and other kinases, regulating transcription, translation, and cytoskeletal dynamics (PubMed:35216969).
  Related references: PMID:35216969
• Subcellular localization includes cytoplasm, nucleus, membrane caveolae, and focal adhesions, reflecting its role in diverse signaling compartments.
• Key post-translational modifications include phosphorylation, acetylation, and ubiquitination; these regulate its activity, localization, and stability.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning the N-terminal region (aa 1-100) of human ERK1; consider this when planning epitope-mapping or blocking experiments.
• For Western blotting (WB), anticipate a band at approximately 43-44 kDa; note that ERK1 may co-migrate with ERK2 (42 kDa) in some samples.
• Cross-reactivity is reported for human, mouse, and rat; validate the antibody in your specific sample matrix to confirm species reactivity.
• This polyclonal antibody is suitable for applications including WB, IHC-P, IF/ICC, and ELISA; optimize dilution and detection conditions for each assay.
• The antibody has been validated by KD (knockdown) in relevant models per the product name; consider including appropriate positive and negative controls in your experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a complete range of IVD raw materials and sourcing services, connecting diagnostic developers with validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For MAPK/ERK signaling research, this anti-ERK1 polyclonal antibody provides a reliable tool for immunoassays. Our one-stop platform simplifies procurement from concept to clinic, with specialized support for rare‑target material sourcing.