[KD Validated] Anti-EGFR Rabbit PolymAb® for WB, IF/ICC, ELISA - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• EGFR is a receptor tyrosine kinase that binds EGF family ligands and activates multiple downstream signaling cascades, including RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, STAT, and NF-kappa-B modules, to convert extracellular cues into cellular responses like proliferation, survival, and migration. Related references: PMID:10805725, PMID:27153536, PMID:2790960, PMID:11116146
• Known EGFR ligands include EGF, TGFA, AREG, epigen, BTC, epiregulin, and HBEGF; ligand binding induces receptor dimerization and autophosphorylation on key cytoplasmic residues. Related references: PMID:12297049, PMID:15611079, PMID:17909029
• Phosphorylated EGFR recruits adapter proteins like GRB2 and directly phosphorylates substrates such as RGS16 (activating its GTPase activity) and MUC1 (increasing interaction with SRC and beta-catenin), linking to G protein signaling and cell adhesion pathways. Related references: PMID:11602604, PMID:11483589
• Isoform 2 may act as an antagonist of EGF action and is notably expressed in ovarian cancers.
• EGFR localizes predominantly at the cell membrane, but also at the endoplasmic reticulum, Golgi apparatus, nuclear membrane, endosomes, and can be secreted.
• It is ubiquitously expressed in tissues and also functions as a host receptor for hepatitis C virus (HCV) entry, mediating CD81-CLDN1 complex formation.
• EGFR is involved in long-term potentiation and pain hypersensitivity (By similarity), and its dysregulation is associated with various cancers.

Experimental Guidance and Technical Tips

• The immunogen used is recombinant human EGFR protein; users should validate reactivity in their specific sample system (e.g., cell lines with known EGFR expression such as A431).
• For Western blotting, multiple bands may be observed corresponding to full-length EGFR (~134 kDa) and proteolytic fragments (e.g., 44, 69, 77 kDa); these are consistent with reported EGFR processing.
• In immunofluorescence/ICC, expect plasma membrane and endosomal staining; permeabilization may be required to detect intracellular pools.
• This antibody is a recombinant rabbit monoclonal (PolymAb®) offering high batch-to-batch consistency, suitable for quantitative ELISA applications.
• As with any antibody, optimal working concentrations and conditions should be experimentally determined by end-user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted sourcing partner for IVD raw materials, offering a comprehensive portfolio of anti-EGFR antibodies and supporting reagents for developing robust diagnostic assays. Whether you need validated antibody pairs, optimized recombinant monoclonal antibodies, or bulk ancillary reagents, our team ensures reliable access to high-quality materials for cancer biomarker and signal transduction research. Explore our catalog for your EGFR-targeted immunoassay needs.