[KD Validated] Anti-ECHS1 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P30084

Core Product Specifications and Parameters

Biological Background: ECHS1 Function and Localization

• ECHS1 encodes the mitochondrial enoyl-CoA hydratase, also known as short-chain enoyl-CoA hydratase (SCEH), a key enzyme in fatty acid beta-oxidation.
• Catalyzes the hydration of trans-2-enoyl-CoA species to (3S)-3-hydroxyacyl-CoA, essential for the breakdown of short- and medium-chain fatty acids (C4–C16) (PubMed:25125611, PubMed:26251176).
  Related references:
  PMID:25125611
  PMID:26251176
• Exhibits high substrate specificity for crotonyl-CoA and moderate activity toward acryloyl-CoA, 3-methylcrotonyl-CoA, and methacrylyl-CoA (PubMed:26251176).
  Related references:
  PMID:26251176
• Possesses isomerase activity, converting trans-3-enoyl-CoA to trans-2-enoyl-CoA, albeit at a lower rate than its hydratase function.
• Localized to the mitochondrial matrix, consistent with its metabolic role.
• Post-translational modifications include acetylation and phosphorylation; linked to disease variants in neurodegeneration.
• Predominantly expressed in liver, fibroblasts, and muscle, with minimal expression in spleen and kidney.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 28-290 of human ECHS1, excluding the N-terminal mitochondrial targeting peptide (aa 1-27). This design favors recognition of the mature, processed form of the protein.
• For western blot (WB), expect a band at ~31 kDa; validate using a positive control (e.g., liver or fibroblast lysates) and consider including a knockout cell line to confirm specificity.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and permeabilization conditions; endogenous ECHS1 localizes to mitochondria, so co-staining with mitochondrial markers can serve as a control.
• In ELISA, the recombinant immunogen protein can be used as a standard, but assay conditions may require optimization for antibody-antigen binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, including validated monoclonal antibodies like this ECHS1 antibody, along with technical services and consulting to support fatty acid oxidation and mitochondrial metabolism research. Our supply chain capabilities encompass antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, enabling seamless progression from concept to clinical application.