KD Validated Anti-DYNC1H1 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14204

Core Product Specifications and Parameters

Biological Background: Cytoplasmic Dynein 1 Heavy Chain 1 (DYNC1H1) Function and Localization

• DYNC1H1 encodes the heavy chain subunit of cytoplasmic dynein 1, a multi-subunit motor protein complex.
• The protein functions as a microtubule-based motor, driving retrograde transport of vesicles and organelles.
• Dynein exhibits ATPase activity; the force-producing power stroke is associated with ADP release.
• DYNC1H1 plays a critical role in mitotic spindle assembly and metaphase plate congression during cell division. Related references: PMID:27462074
• It localizes predominantly in the cytoplasm and is associated with the cytoskeleton.
• Mutations in DYNC1H1 are linked to neurological disorders including Charcot-Marie-Tooth disease, spinal muscular atrophy, and intellectual disability.
• The protein undergoes post-translational modifications such as acetylation and phosphorylation, as identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-210) of human DYNC1H1; the antibody may recognize epitopes within this domain.
• Due to the large molecular weight of DYNC1H1 (~532 kDa), for Western blotting consider using low-percentage SDS-PAGE gels (e.g., 6% or gradient) and extended transfer times.
• For immunohistochemistry and immunofluorescence, appropriate fixation and antigen retrieval methods should be optimized; validating the antibody in knockdown or knockout models is recommended to confirm specificity.
• The recombinant protein immunogen can serve as a positive control in ELISA and other quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material supplier, CamelBio bridges research and diagnostic development by providing high-quality reagents for targets like DYNC1H1 involved in intracellular transport and neurological disorders. We offer validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for sourcing rare targets. Partner with CamelBio to streamline your assay development from concept to clinical application.