KD Validated Anti-DNMT1 Rabbit Monoclonal Antibody for WB, IP, ELISA - P26358

Core Product Specifications and Parameters

Biological Background: DNMT1 Function and Localization

• DNA (cytosine-5)-methyltransferase 1 is responsible for methylating CpG dinucleotides, with a strong preference for hemimethylated DNA substrates.
  Related references: PMID:21745816, PMID:26070743

• During S phase, DNMT1 associates with DNA replication sites to copy methylation patterns onto the newly synthesized strand, ensuring epigenetic inheritance.
  Related references: PMID:17200670, PMID:21745816

• In G2 and M phases, it remains chromatin-bound to maintain DNA methylation independently of replication.
  Related references: PMID:21745816

• It coordinates DNA methylation with histone modifications such as histone H3 methylation, integrating epigenetic signals.
  Related references: PMID:16357870

• DNMT1 mediates transcriptional repression through direct interaction with HDAC2.
  Related references: PMID:10888872

• Together with DNMT3B and the CTCFL/BORIS factor, it activates BAG1 gene expression by modulating histone H3 dimethylation at specific residues.
  Related references: PMID:18413740

• In colorectal cancer cells, DNMT1 forms a corepressor complex with activated KRAS to hypermethylate and silence tumor suppressor genes.
  Related references: PMID:24623306

• The protein localizes to the nucleus and chromosomes, and is ubiquitously expressed, with high levels in fetal tissues, heart, kidney, and placenta. It exists as multiple isoforms due to alternative splicing, with isoform 2 being less abundant.

Experimental Guidance and Technical Tips

• The immunogen is designed from the N-terminal region (amino acids 1–100) of human DNMT1; antibodies generated from this region may recognize epitopes conserved across species, consistent with the observed cross-reactivity with mouse and rat.
• DNMT1 has a predicted molecular weight of approximately 183 kDa; ensure appropriate gel percentage and transfer conditions in western blotting to resolve high molecular weight proteins effectively.
• The antibody is a rabbit monoclonal, which typically offers high specificity and lot-to-lot consistency; consider validating the clone in your specific sample type and application to confirm performance.
• Cross-reactivity data support use in human, mouse, and rat models, but individual protein expression levels and post-translational modifications may vary; pilot experiments with appropriate controls are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in supplying high-quality IVD raw materials to diagnostic manufacturers, laboratories, and research institutes. This DNMT1 antibody supports research into DNA methylation, epigenetic regulation, and cancer biology. As a one-stop procurement bridge, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare target raw materials, streamlining your journey from concept to clinic.