[KD Validated] Anti-DEC1 Rabbit Monoclonal Antibody for WB, ELISA - O14503

Core Product Specifications and Parameters

Biological Background: DEC1 Function and Localization

• BHLHE40 (Class E basic helix-loop-helix protein 40), commonly known as DEC1, SHARP-2, or STRA13, is a transcriptional repressor with a central role in circadian rhythm regulation.
• It acts as the negative limb of the DEC autoregulatory feedback loop, repressing the CLOCK-BMAL1 heterodimer by competing for E-box elements (5'-CACGTG-3') within target gene promoters, thereby controlling clock and clock-controlled genes. Related references: PMID:12397359 PMID:18411297 PMID:14672706 PMID:15560782
• The DEC loop is interlocked with the PER/CRY loop: DEC1 represses PER1/2 expression and is itself repressed by PER1/2 and CRY1/2. Related references: PMID:15193144
• DEC1 may regulate chondrocyte differentiation through the cAMP signaling pathway. Related references: PMID:19786558
• It functions as a corepressor of RXR and RXR-LXR heterodimers, attenuating ligand-induced transactivation of RXRA and NR1H3/LXRA. Related references: PMID:19786558
• Drives the circadian rhythm of blood pressure via transcriptional repression of ATP1B1 in the cardiovascular system. Related references: PMID:30012868
• Subcellular localization is primarily nuclear and cytoplasmic.
• Expressed in cartilage, spleen, intestine, lung, and to a lesser extent in heart, brain, liver, muscle, and stomach.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 121–300 of human DEC1 (NP_003661.1), likely spanning functionally important regions; consider epitope accessibility when designing assays.
• For Western blot, a band near 46 kDa is expected; UniProt lists a full-length molecular weight of ~45.5 kDa, and slight shifts may occur due to phosphorylation or other modifications.
• This antibody is recommended for WB and ELISA applications; if using in other techniques, validate performance in the relevant sample system.
• Reported cross-reactivity is for human samples; for non-human species, sequence homology analysis is advised prior to use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive, one-stop access to IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. For circadian rhythm and transcriptional regulation research, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development and production scale-up.