KD Validated Anti-Cyclophilin F Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P30405

Core Product Specifications and Parameters

Biological Background: Cyclophilin F Function and Localization

• Cyclophilin F (PPIF), also known as Cyclophilin D, CypD, Cyp-M, or mitochondrial cyclophilin, is encoded by the PPIF gene and localizes to the mitochondrial matrix.
• It functions as a peptidyl-prolyl cis-trans isomerase (PPIase), catalyzing the isomerization of proline imidic peptide bonds and assisting in protein folding. Related references: PMID:20676357
• Cyclophilin F is involved in the regulation of the mitochondrial permeability transition pore (mPTP), where it may promote pore opening leading to apoptosis or necrosis; the requirement of its PPIase activity for this function remains debated. Related references: PMID:26387735
• In cooperation with mitochondrial p53/TP53, Cyclophilin F activates oxidative stress-induced necrosis. Related references: PMID:22726440
• It exhibits anti-apoptotic activity independently of mPTP and, in collaboration with BCL2, inhibits cytochrome c-dependent apoptosis. Related references: PMID:19228691
• Cyclophilin F is subject to post-translational modifications including acetylation and S-nitrosylation, and alternative splicing generates multiple isoforms, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide corresponding to amino acids 108-207 of human Cyclophilin F (NP_005720.1); consider this epitope region when designing overlapping peptides or truncation mutants.
• The product is designated as KD Validated, suggesting potential utility in knockdown verification experiments; however, users should independently validate performance in their specific cellular models.
• For Western blotting (WB), a band near the reported molecular weight of 22 kDa is expected; optimize loading amounts and detection conditions to confirm specificity.
• For immunohistochemistry (IHC-P), antigen retrieval method and primary antibody dilution should be empirically determined; include appropriate positive and negative tissue controls.
• For ELISA, this monoclonal antibody can be employed as a capture or detection reagent; validate pairing with a compatible secondary antibody and assay matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-materials partner, offering this Cyclophilin F antibody alongside a broad portfolio of mitochondrial research reagents, validated antibody pairs, and ancillary materials. Our one-stop sourcing model simplifies procurement for assay developers, from early R&D to clinical application. Reach out to explore bulk supplies, rare-target sourcing, and tailored solutions for your diagnostic and mitochondrial apoptosis/necrosis research needs.