[KD Validated] Anti-CYB5R3 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P00387

Core Product Specifications and Parameters

Biological Background: CYB5R3 Function and Localization

• CYB5R3 encodes NADH-cytochrome b5 reductase 3, also known as diaphorase-1 (DIA1), a flavoprotein oxidoreductase.
• Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor, playing a role in electron transport.
• Localizes to the endoplasmic reticulum membrane, mitochondrial outer membrane, and cytoplasm, indicating diverse subcellular functions.
• Expressed at late stages of erythroid maturation, suggesting a role in red blood cell development.
• Involved in cholesterol and steroid biosynthesis and metabolism pathways, as indicated by associated keywords (Cholesterol biosynthesis, Steroid metabolism).
• Subject to alternative promoter usage and alternative splicing, leading to multiple transcript variants.
• Post-translationally modified by acetylation, phosphorylation, and myristoylation, which may regulate its activity and localization.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 27-301, covering most of the protein, which may provide recognition of multiple epitopes.
• For Western blot, a band around 34 kDa is expected; validate with appropriate positive and negative controls.
• Given knockdown validation, this antibody is suitable for assessing CYB5R3 protein levels after siRNA or CRISPR-mediated gene silencing.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider optimizing antigen retrieval and permeabilization conditions based on tissue and cell type.
• Cross-reactivity with mouse and rat allows use in rodent models, but verify expected band size in those species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to specialized IVD raw materials, including this anti-CYB5R3 monoclonal antibody for investigating electron transport and metabolic disease mechanisms. Beyond single antibodies, CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal panels, and ancillary reagents for assay development. For researchers targeting rare or emerging biomarkers, CamelBio offers tailored raw-material sourcing to bridge discovery and clinical application.