[KD Validated] Anti-CREBBP Rabbit Monoclonal Antibody for WB/IF/IHC - Q92793

Core Product Specifications and Parameters

Biological Background: CREB-binding protein (CREBBP) Function and Localization

• CREBBP (also known as CBP, KAT3A) is a histone lysine acetyltransferase and transcriptional coactivator encoded by the CREBBP gene.
• Mediates acetylation of histone H3 at Lys18 and Lys27 (H3K18ac and H3K27ac), creating specific tags for transcriptional activation. Related references: PMID:21131905, PMID:24616510
• Acetylates non-histone proteins such as PCNA (promoting its removal during nucleotide excision repair) and POLR1E (decreasing RNA polymerase I association with rDNA). Related references: PMID:24939902, PMID:24207024
• Acts as a circadian transcriptional coactivator, enhancing the activity of NPAS2-BMAL1 and CLOCK-BMAL1 heterodimers. Related references: PMID:14645221
• In addition to acetyl-CoA, can use lactoyl-CoA to mediate protein lactylation, as seen with MRE11 in response to DNA damage. Related references: PMID:38128537
• Localizes to both the cytoplasm and nucleus, consistent with its diverse roles in transcription and repair.
• Contains multiple conserved domains including a bromodomain, zinc fingers, and a histone acetyltransferase (HAT) domain, characteristic of the p300/CBP family.

Experimental Guidance and Technical Tips

• For WB, use a low-percentage gel (e.g., 6-8%) and optimized transfer for high-molecular-weight protein (265 kDa).
• For IF/ICC, fix cells with 4% PFA and permeabilize with 0.1% Triton X-100; consider blocking with 5% BSA.
• For IHC-P, perform heat-mediated antigen retrieval in citrate buffer (pH 6.0); test antibody dilution series.
• Validate reactivity in the appropriate cell lines or tissues; the antibody is reported to cross-react with human, mouse, and rat.
• The immunogen (amino acids 2343–2442) targets a C-terminal region; be aware of potential isoforms.

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CamelBio offers a one-stop procurement solution for IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies like this anti-CREBBP clone, and bulk ancillary reagents. Whether you are developing diagnostic assays for epigenetic targets or require rare target sourcing, our supply chain supports research and clinical transition from concept to clinic. Complement your project with our technical consulting and custom antibody development services.