[KD Validated] Anti-COX IV Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P13073

Core Product Specifications and Parameters

Biological Background: COX IV Function and Localization

• COX IV isoform 1 (COX4I1) is a nuclear-encoded subunit of cytochrome c oxidase (Complex IV), the terminal enzyme of the mitochondrial electron transport chain.
• Complex IV catalyzes the reduction of molecular oxygen to water, coupling this reaction to proton translocation across the inner mitochondrial membrane to generate the proton gradient for ATP synthesis.
• The COX4I1 gene belongs to the COX4 family, with isoforms arising from alternative splicing; COX IV-1 is the ubiquitously expressed isoform.
• The protein localizes to the mitochondrial inner membrane, with a transmembrane helix anchoring it within the lipid bilayer.
• Post-translational modifications include acetylation and phosphorylation, which may regulate enzyme activity or assembly.
• Mutations in COX4I1 are associated with primary mitochondrial disease, particularly cytochrome c oxidase deficiency (MC4DN16), underscoring its critical role in oxidative phosphorylation.
• COX IV is expressed across many tissues, consistent with the ubiquitous requirement for aerobic respiration.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 23-98) of human COX IV; consider using siRNA-mediated knockdown (e.g., [KD Validated]) to confirm antibody specificity in your sample system.
• For Western blotting, COX IV migrates at approximately 20 kDa; mitochondrial-enriched fractions or high-respiratory tissue lysates may yield stronger signals.
• In immunohistochemistry or immunofluorescence, combine with mitochondrial counterstains or optimize permeabilization to improve detection of this membrane-bound antigen.
• When used in ELISA, verify binding specificity by competitive inhibition with recombinant COX IV protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material procurement, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For mitochondrial research and respiratory chain studies, we provide reliable access to high-quality COX IV antibodies and related targets. Reach out to our team for custom sourcing and technical consultation.