[KD Validated] Anti-CD98/SLC3A2 Rabbit Monoclonal Antibody for WB, IF/ICC, FC, ELISA - P08195

Core Product Specifications and Parameters

Biological Background: CD98/SLC3A2 Function and Localization

• SLC3A2 (4F2hc, CD98) is a type II transmembrane glycoprotein that serves as the heavy chain for multiple heterodimeric amino acid transporters, partnering with light chains of the SLC7 family (SLC7A5–SLC7A11). Related references: PMID:10574970 PMID:10903140 PMID:11557028

• The SLC3A2-SLC7A5 (LAT1) heterodimer mediates sodium-independent transport of large neutral amino acids and thyroid hormones, and is crucial for mTORC1 activation via leucine uptake in lysosomes. Related references: PMID:10574970 PMID:11389679 PMID:25998567

• SLC3A2/SLC7A11 (xCT) exchanges extracellular cystine for intracellular glutamate, supporting glutathione synthesis and redox balance. Related references: PMID:34880232

• Beyond transport, SLC3A2 modulates integrin signaling, promoting cell spreading, migration, and tumor progression. Related references: PMID:11121428 PMID:15625115

• SLC3A2 acts as a receptor for hepatitis C virus (HCV) entry via the SLC7A5 complex, and for malaria parasite Plasmodium vivax in immature red blood cells.

• Ubiquitously expressed with highest levels in kidney, placenta, and testis, SLC3A2 localizes to the plasma membrane (apical/basolateral), lysosomes, melanosomes, and cell junctions.

• Keywords: Acetylation, Disulfide bond, Glycoprotein, Phosphoprotein, Ubl conjugation – suggesting extensive post-translational regulation.

Experimental Guidance and Technical Tips

• The recombinant immunogen (aa 206-630) corresponds to the large ectodomain of SLC3A2, which is well-conserved; the antibody is expected to detect the target in denatured (WB) and native (IF/ICC, FC) conditions.
• For Western blot, multiple bands around 57–71 kDa are observed due to heterogeneous N-glycosylation; treatment with PNGase F can simplify band pattern.
• For immunofluorescence, fixation and permeabilization protocols (e.g., methanol or Triton X-100) may be necessary to access intracellular SLC3A2 pools in lysosomes.
• Provided cross-reactivity is human; for other species, verify sequence homology of the immunogen region and perform a species-specific validation.
• For flow cytometry, the antibody can be used to detect cell surface SLC3A2; consider using a live-cell staining protocol with appropriate isotype controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material partner, streamlining the sourcing of high-quality antibodies, antigens, and ancillary reagents for diagnostic and life science workflows. The CD98/SLC3A2 antibody fits into cancer biology, amino acid transport, and infectious disease research—supporting the development of diagnostic assays and therapeutic monitoring. Whether you need bulk monoclonal antibodies, matched antigen controls, or technical consultation, CamelBio bridges your concept-to-clinic journey.