[KD Validated] Anti-CCNT1 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, ELISA - O60563

Core Product Specifications and Parameters

Biological Background: Cyclin-T1 Function and Localization

• Cyclin-T1 is the regulatory subunit of the positive transcription elongation factor b (P-TEFb) complex, which consists of CDK9 and cyclin T1. P-TEFb phosphorylates the C-terminal domain (CTD) of RNA polymerase II to promote productive transcriptional elongation (PubMed:16109376, PubMed:16109377, PubMed:30134174). Related references: PMID:16109376, PMID:16109377, PMID:30134174
• Cyclin-T1 activates CDK9 by mediating liquid-liquid phase separation (LLPS), which enhances P-TEFb binding to the CTD of RNA Pol II (PubMed:29849146, PubMed:35393539). Related references: PMID:29849146, PMID:35393539
• During HIV-1 or SIV infection, Cyclin-T1 binds the viral transactivator Tat, increasing its affinity for TAR RNA and serving as an essential cofactor for viral gene transcription.
• Cyclin-T1 is ubiquitously expressed and localizes to the nucleus.
• The protein undergoes various post-translational modifications, including phosphorylation, acetylation, and ADP-ribosylation, which regulate its activity.
• Alternative splicing of the CCNT1 gene generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 621–720 of human Cyclin T1, targeting the C‑terminal region.
• This antibody has been shown to detect human Cyclin T1 in Western blot, immunoprecipitation, immunofluorescence, and ELISA applications; consider optimizing for your specific experimental conditions.
• Validate the antibody in your relevant sample system and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with a comprehensive range of IVD raw materials, including validated monoclonal antibodies such as this Cyclin-T1 antibody. Our supply capabilities cover antibody pairs, bulk ancillary reagents, and custom sourcing for transcriptional regulation and virology research targets. Let CamelBio streamline your raw-material procurement from concept to clinic.