[KD Validated] Anti-CAT1 Rabbit Polyclonal Antibody for WB - P30825

Core Product Specifications and Parameters

Biological Background: CAT1 Function and Localization

• CAT1, also known as SLC7A1, is a high-affinity, low-capacity cationic amino acid transporter (permease) that mediates the uptake of arginine, lysine, and ornithine in non-hepatic tissues.
• It functions as an ecotropic retroviral leukemia receptor, serving as a cell surface receptor for ecotropic murine leukemia viruses (hence the alternate names ERR and REC1L).
• The protein is a member of the solute carrier family 7 (SLC7) and contains multiple transmembrane domains, consistent with its role in membrane transport.
• CAT1 is ubiquitously expressed across human tissues, ensuring systemic availability of essential cationic amino acids.
• Subcellularly, it localizes to the cell membrane, where it facilitates the influx of cationic amino acids from the extracellular environment into the cytoplasm.
• The protein is glycosylated and has a molecular weight of approximately 68 kDa as determined by SDS-PAGE (calculated mass: 67.6 kDa).

Experimental Guidance and Technical Tips

• For Western blot, consider using a dilution range of 1:500–1:2000; a specific band around 68 kDa is expected based on the predicted molecular weight and endogenous glycosylation.
• The antibody may also detect mouse CAT1 due to reported cross-reactivity; suitable positive controls include human and mouse cell lysates.
• Antigen retrieval is not required for Western blotting; standard SDS-PAGE and transfer conditions are typically adequate.
• In ELISA applications, coat the synthetic peptide immunogen or recombinant protein and validate the antibody’s binding affinity under your specific assay conditions.
• This antibody is not recommended for immunohistochemistry, as it has only been validated for WB and ELISA.
• For optimal results, validate the antibody in a pilot experiment in your sample matrix, using appropriate negative and positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials and technical services, covering every stage from concept to clinic. This anti-CAT1 polyclonal antibody supports research into amino acid transport, viral receptor biology, and related disease areas. We leverage a robust supply network to offer validated antibody pairs, optimized polyclonals, bulk ancillary reagents, and rare target raw-material sourcing—providing flexible procurement solutions to accelerate your IVD development.