[KD Validated] Anti-Casein Kinase 2 alpha (CSNK2A1) Rabbit Monoclonal Antibody for WB, ELISA - P68400

Core Product Specifications and Parameters

Biological Background: Casein Kinase 2 alpha Function and Localization

• CSNK2A1 encodes the catalytic subunit α of casein kinase II (CK2), a constitutively active serine/threonine-protein kinase complex that phosphorylates substrates with acidic residues C-terminal to the phosphoacceptor site, regulating cell cycle progression, apoptosis, and transcription (PubMed:11239457, PubMed:11704824, PubMed:16193064).
  Related references: PMID:11239457 PMID:11704824 PMID:16193064
• CK2α acts in the p53/TP53-dependent spindle assembly checkpoint to maintain G2 arrest upon spindle damage and phosphorylates p53 at Ser-392 after UV irradiation to promote apoptosis (PubMed:11704824, PubMed:19188443, PubMed:11239457).
  Related references: PMID:11704824 PMID:19188443 PMID:11239457
• It phosphorylates DNA repair proteins (MDC1, MRE11, RAD9A, RAD51, HTATSF1) and facilitates their recruitment to DNA damage sites (PubMed:18411307, PubMed:18583988, PubMed:18678890).
  Related references: PMID:18411307 PMID:18583988 PMID:18678890
• CK2α regulates apoptosis by phosphorylating CASP9, CASP2, and NOL3, protecting CASP9 from cleavage and inhibiting CASP2 dimerization; it also phosphorylates YY1 to prevent cleavage by CASP7 (PubMed:16193064, PubMed:22184066).
  Related references: PMID:16193064 PMID:22184066
• As a transcriptional regulator, it phosphorylates RNA polymerases I, II, III, and IV and transcription factors (NF-kB, STAT1, CREB1, MYC, JUN, FOS), modulates Wnt signaling by phosphorylating CTNNB1 and LEF1, and influences circadian rhythm through BMAL1 phosphorylation (PubMed:12631575, PubMed:19387550, PubMed:19387551, PubMed:19387549).
  Related references: PMID:12631575 PMID:19387550 PMID:19387551 PMID:19387549
• CK2α localizes predominantly to the nucleus and is expressed in gastric carcinoma tissue, with levels increasing with carcinoma progression (at protein level).
• Additional functions include ectokinase activity phosphorylating extracellular proteins, roles in viral life cycles (EBV, HSV, HBV, etc.), and regulation of chaperone function via Hsp90 phosphorylation (PubMed:12631575, PubMed:19387550, PubMed:19387551, PubMed:30699359).
  Related references: PMID:12631575 PMID:19387550 PMID:19387551 PMID:30699359

Experimental Guidance and Technical Tips

• A synthetic N-terminal peptide (aa1-100) immunogen yields a monoclonal antibody that recognizes the full-length CSNK2A1 protein; perform peptide blocking to confirm specificity if needed.
• For Western blotting, prepare whole-cell or nuclear lysates under reducing conditions; a band at 45 kDa is expected. Include a positive control known to express CK2α, such as HeLa or HEK293 cells.
• For ELISA, the antibody may be suitable for capture or detection depending on assay format; validate pairing with a detection antibody if used in sandwich ELISA.
• Cross-reactivity is reported for human; homology with mouse and rat is high, but cross-reactivity should be verified experimentally.
• Storage and handling: Ship on ice bag; upon receipt, aliquot and store at -20°C or -80°C for long-term. Avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, providing diagnostic manufacturers and researchers with validated monoclonal antibodies like this casein kinase 2 alpha antibody for kinase signaling and cancer research applications. We offer bulk supply, custom antibody development, and supplementary reagents to accelerate your assay development — from concept to clinic. Contact our team to discuss your CK2 pathway sourcing needs.