KD Validated Anti-BUB1 Monoclonal Antibody for WB, ELISA - O43683

Core Product Specifications and Parameters

Biological Background: BUB1 Function and Localization

• Mitotic checkpoint serine/threonine-protein kinase, also known as BUB1A, encoded by the BUB1 gene. Essential for the spindle-assembly checkpoint (SAC) and correct chromosome alignment during mitosis.
• Required for the kinetochore localization of multiple checkpoint proteins including CENPF, BUB1B, CENPE, MAD2L1, PLK1, and AURKB, thereby ensuring robust SAC signaling.
• Forms a complex with BUB3 that inhibits the anaphase-promoting complex/cyclosome (APC/C) by phosphorylating its activator CDC20; also phosphorylates MAD1L1 and BUB3.
• Promotes centromeric localization of TOP2A, contributing to proper chromosome segregation. Related references: PMID:35044816
• Plays a critical role in defining the localization of SGO1, thereby affecting sister chromatid cohesion and chromosome segregation.
• Acts as a substrate for APC/C-Cdh1 and mediates cell death in response to chromosome missegregation, suppressing spontaneous tumorigenesis.
• Localizes to the nucleus, concentrated at the centromere and kinetochore on chromosomes during mitosis.
• Highly expressed in testis and thymus; lower expression in colon, spleen, lung, and small intestine. Associated with tissues having a high mitotic index. Keywords: 3D-structure, Alternative splicing, Apoptosis, ATP-binding, Cell cycle, Cell division, Centromere, Chromosome, Chromosome partition, Host-virus interaction, Intellectual disability, Kinase, Kinetochore, Mitosis, Nucleotide-binding, Nucleus, Phosphoprotein, Primary microcephaly, Serine/threonine-protein kinase, Transferase, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen is an N-terminal fragment (aa 1-150) of human BUB1; confirm specificity for full-length protein and potential splice variants in your system using appropriate controls.
• For Western blot, expect bands around 115–122 KDa due to multiple isoforms; validate with molecular weight markers and consider using the KD-validated status as supporting evidence of specificity.
• ELISA performance may depend on the coating antigen format; optimize coating conditions and antibody titration for best signal-to-noise ratio.
• Cross-reactivity with other species has not been reported; if extending to other species, verify sequence homology and perform experimental validation.

CamelBio: Your One-Stop Sourcing Bridge

This BUB1 antibody supports mitosis and cell cycle research, ideal for cancer diagnostic development. CamelBio provides diagnostic manufacturers and labs with one-stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing—streamlining your path from concept to clinic.