[KD Validated] Anti-Bcl-2 Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - P10415

Core Product Specifications and Parameters

Biological Background: Bcl-2 Function and Localization

• Encoded by the BCL2 gene, Bcl-2 undergoes alternative splicing to produce multiple isoforms.
• Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Related references: PMID:1508712, PMID:8183370
• Regulates cell death by controlling mitochondrial membrane permeability, functioning in a feedback loop with caspases, and inhibiting caspase activity by preventing cytochrome c release and/or binding APAF-1. Related references: PMID:11368354
• Acts as an inhibitor of autophagy by interacting with BECN1 and AMBRA1 during non-starvation conditions. Related references: PMID:18570871, PMID:20889974, PMID:21358617
• May attenuate inflammation by impairing NLRP1-inflammasome activation, thereby inhibiting CASP1 activation and IL1B release. Related references: PMID:17418785
• Localizes to the mitochondrion outer membrane, nucleus membrane, endoplasmic reticulum membrane, and cytoplasm.
• Expressed in a variety of tissues.
• Undergoes post-translational modifications including phosphorylation and ubiquitination.
• Associated with chromosomal rearrangements in cancer and disease variants; classified as a proto-oncogene.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–211 of human Bcl-2, covering the BH4, BH3, BH1, and BH2 domains; this design may contribute to broad application reactivity.
• For Western blot, the predicted molecular weight is 26 kDa; validate in the relevant sample system, as post-translational modifications may cause band shifts.
• Reported for use in WB, IHC-P, IP, and ELISA; consider optimizing antibody dilutions and conditions for each specific assay.
• Given confirmed cross-reactivity with human and mouse, this antibody is suitable for comparative studies in these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This [KD Validated] Bcl-2 rabbit mAb supports apoptosis and autophagy research, making it a valuable tool for oncology and cell death studies. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.