[KD Validated] Anti-Aurora B Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q96GD4

Core Product Specifications and Parameters

Biological Background: Aurora kinase B Function and Localization

• Aurora kinase B (AURKB, also known as AIK2, AIM1, STK12) is a serine/threonine kinase that serves as the catalytic subunit of the chromosomal passenger complex (CPC), which is essential for accurate chromosome segregation and cytokinesis (PubMed:11516652, PubMed:12925766, PubMed:14610074). Related references: PMID:11516652 PMID:12925766 PMID:14610074
• It regulates key mitotic events by phosphorylating substrates such as BIRC5/survivin, INCENP, histones H3S10/H3S28, and CENPA, ensuring spindle assembly checkpoint activation and kinetochore-microtubule attachment (PubMed:11784863, PubMed:11856369, PubMed:11756469). Related references: PMID:11784863 PMID:11856369 PMID:11756469
• AURKB activity is potentiated by INCENP phosphorylation via a positive feedback loop with HASPIN at centromeres (PubMed:11516652, PubMed:21658950). Related references: PMID:11516652 PMID:21658950
• The kinase is a critical component of the cytokinesis checkpoint, phosphorylating CHMP4C to delay abscission and prevent chromosome bridge formation (PubMed:22422861, PubMed:24814515). Related references: PMID:22422861 PMID:24814515
• During mitosis, AURKB localizes dynamically to the nucleus, centromeres, kinetochores, spindle microtubules, and the midbody, reflecting its multiple roles in chromosome dynamics and cell division.
• It is highly expressed in thymus, spleen, lung, testis, colon, placenta, and fetal liver, and its expression peaks during S and G2/M phases; it is frequently overexpressed in cancers, with high levels in metastatic versus normal liver.
• AURKB phosphorylates histone H3 at Ser10 and Ser28, a hallmark of mitosis and a commonly used marker for cell cycle studies (PubMed:11784863, PubMed:11856369). Related references: PMID:11784863 PMID:11856369
• Additional substrates include KIF2C, RACGAP1, VIM, and p53/TP53, linking AURKB to diverse mitotic processes and checkpoint control (PubMed:20959462). Related references: PMID:20959462

Experimental Guidance and Technical Tips

• The immunogen used is an N-terminal peptide (amino acids 1–100) of human Aurora B; for Western blot, denaturing conditions may enhance epitope exposure. Non‑specific bands may be minimized by optimizing blocking and antibody dilution.
• In IF/ICC, Aurora B exhibits dynamic localization during mitosis; fixation and permeabilization protocols should be validated to preserve antigenicity and access to centromeres/midbody.
• For ELISA, consider using the antibody for capture or detection after confirming binding to recombinant or native Aurora B, with appropriate titration and control wells.
• KD (knockdown) validation suggests specificity; however, confirm reactivity in your sample system using appropriate negative (e.g., knockout) controls.
• Since the antibody cross‑reacts with mouse and rat, it can be employed in rodent cell lines and tissues, though species‑specific validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material procurement, connecting diagnostic developers with validated antibodies, recombinant proteins, and ancillary reagents from discovery to commercialization. Our portfolio supports mitosis and cancer biomarker research with high‑quality, reproducible tools. For anti‑Aurora B antibodies and rare‑target sourcing, CamelBio delivers tailored solutions to streamline your assay development pipeline.