KD Validated Anti-ATF1 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P18846

Core Product Specifications and Parameters

Biological Background: ATF1 Function and Localization

• ATF-1 (Activating transcription factor 1) is a cAMP-dependent transcription factor that binds the canonical cAMP response element (CRE) consensus sequence 5'-GTGACGT[AC][AG]-3'.
• It also binds the Tax-responsive element (TRE) of HTLV-I, mediating PKA-induced stimulation of CRE-reporter genes.
• ATF-1 represses the expression of antioxidant detoxification genes such as FTH1, linking it to oxidative stress responses.
• It triggers cell proliferation and transformation, playing a role in oncogenesis.
• ATF-1 localizes to the nucleus and functions as a DNA-binding transcriptional activator.
• The protein undergoes alternative splicing and can be involved in chromosomal rearrangements (e.g., EWS-ATF1, FUS/ATF-1 fusions).
• It is a phosphoprotein subject to ubiquitin-like conjugation (isopeptide bond), highlighting multiple post-translational regulatory mechanisms.

Experimental Guidance and Technical Tips

• The antibody is generated against recombinant protein, providing high specificity; performance should be validated in your specific experimental context.
• In Western blotting (WB), a clear band at approximately 29kDa is expected. Consider using appropriate positive and negative controls.
• For immunofluorescence (IF/ICC), nuclear staining is anticipated given ATF-1's subcellular localization. Validate in relevant cell lines with known expression.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and antibody concentration using tissue specimens with documented ATF-1 expression.
• Cross-reactivity with mouse and rat species allows for use in common model organisms, but confirm reactivity in your specific models.
• When performing ELISA, consider pairing with a capture antibody if developing a sandwich assay, and validate linearity and sensitivity.

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CamelBio provides total solutions for IVD raw-material sourcing—from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target development. For researchers and manufacturers focusing on transcription factors like ATF1, CamelBio streamlines procurement by offering access to quality-assured reagents and customizable support, ensuring a reliable supply for diagnostics and therapeutic research.