[KD Validated] Anti-ADAR1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P55265

Core Product Specifications and Parameters

Biological Background: ADAR1 Function and Localization

• ADAR1 (also known as DRADA, p136, IFI-4) is a double-stranded RNA-specific adenosine deaminase that catalyzes the conversion of adenosine to inosine in dsRNA (A-to-I RNA editing). This post-transcriptional modification can alter codon recognition, splicing, RNA stability, and structure.
  Related references:
  PMID:12618436
  PMID:7565688
  PMID:7972084

• The protein plays a critical role in both cellular and viral RNA metabolism. Cellular substrates include neurotransmitter receptors (GRIA2, HTR2C, GABRA3) and the bladder cancer-associated protein BLCAP, where editing modifies amino acid sequences and functional properties.

• ADAR1 exhibits dual roles in viral infection: it can exert antiviral effects (e.g., inhibiting HCV replication via hyper-editing) or proviral effects (e.g., enhancing HDV, MV, VSV, and HIV-1 replication). Some effects are editing-independent, such as suppression of the antiviral kinase PKR/EIF2AK2.

• ADAR1 is ubiquitously expressed, with the highest levels in brain and lung. Isoform 5 is overexpressed in astrocytomas and its expression increases with tumor grade.
  Related references:
  PMID:7972084

• Subcellularly, ADAR1 localizes to the cytoplasm, nucleus, and nucleolus, reflecting its diverse roles in RNA processing and innate immunity.

• Post-translational modifications include phosphorylation, methylation, and ubiquitination, and it contains zinc-binding and RNA-binding domains. Mutations in ADAR1 are associated with Aicardi-Goutières syndrome (AGS6), an autoimmune disorder.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 610–810 of human ADAR1, targeting the C-terminal region. For Western blotting, the predicted molecular weight is ~136 kDa, but isoform-specific bands may be observed; validate using known positive controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider optimizing antigen retrieval and antibody dilution using tissues with known high expression (e.g., brain or lung). Co-staining with organelle markers may help confirm cytoplasmic or nuclear localization.
• In ELISA applications, pair with a suitable capture or detection antibody and validate in the relevant sample matrix to ensure specificity for ADAR1.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated supplier of high-quality IVD raw materials and technical solutions, supporting diagnostic developers from concept to clinic. This anti-ADAR1 monoclonal antibody, targeted to RNA editing and innate immunity research, represents just one of many ready-to-use or customizable reagents we offer. Beyond monoclonal antibodies, we provide validated antibody pairs, antigen proteins, and bulk ancillary reagents—streamlining procurement for diagnostic manufacturing, assay development, and translational research.