[KD Validated] Anti-ACSL4 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - O60488

Core Product Specifications and Parameters

Biological Background: ACSL4 Function and Localization

• ACSL4 (acyl-CoA synthetase long-chain family member 4) is a long-chain-fatty-acid--CoA ligase that catalyzes the conversion of long-chain fatty acids into their active acyl-CoA derivatives, critical for both lipid biosynthesis and β-oxidation. Related references: PMID:21242590 PMID:22633490 PMID:24269233
• The enzyme preferentially activates arachidonate and eicosapentaenoate, and also processes epoxyeicosatrienoic acids (EETs), thereby modulating glucose-stimulated insulin secretion and prostaglandin E2 secretion. Related references: PMID:21242590
• ACSL4 is a key mediator of ferroptosis by activating polyunsaturated fatty acids (especially arachidonate and adrenate) to form lipid-peroxidation substrates. Related references: PMID:27842070 PMID:35027735 PMID:38720107
• The protein is localized to the mitochondrial outer membrane, endoplasmic reticulum membrane, and cell membrane.
• ACSL4 belongs to the long-chain acyl-CoA synthetase family and is also known as ACS4, FACL4, LACS4.
• The predicted molecular weight is approximately 79 kDa, and post-translational modifications include phosphorylation.
• Genetic variations in ACSL4 are associated with Alport syndrome, intellectual disability, and hereditary hemolytic anemia.

Experimental Guidance and Technical Tips

• Based on the synthetic peptide immunogen, the antibody is expected to recognize both native and denatured ACSL4, making it suitable for WB and ELISA.
• For IF/ICC and IHC-P, optimize fixation and antigen retrieval conditions due to the protein’s membrane localization.
• Given the role of ACSL4 in ferroptosis, consider validating the antibody in cell models treated with ferroptosis inducers.
• The antibody cross-reacts with mouse and rat ACSL4, facilitating multi-species studies; verify reactivity in your specific tissue or cell line.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw material partner, CamelBio offers a wide range of validated antibodies and ancillary reagents, supporting research areas such as lipid metabolism and ferroptosis. This ACSL4 antibody is part of our optimized monoclonal antibody portfolio, and we can assist with sourcing rare target raw materials and custom antibody services. From initial concept to clinical applications, CamelBio connects you with high-quality tools to accelerate your diagnostic development.