KD Validated Anti-ABCA6 Rabbit Polyclonal Antibody for WB and ELISA - Q8N139

Core Product Specifications and Parameters

Biological Background: ABCA6 Function and Localization

• ABCA6 (ATP-binding cassette sub-family A member 6, also known as EST155051) is a probable transporter implicated in macrophage lipid transport and homeostasis.
• It belongs to the ABC transporter family, characterized by ATP-binding domains and transmembrane helices, functioning as a translocase.
• ABCA6 localizes to the Golgi apparatus membrane, suggesting a role in intracellular lipid trafficking.
• The protein is widely expressed in human tissues, with relatively higher expression in the liver.
• Post-translational modifications include glycosylation (glycoprotein) and alternative splicing, yielding multiple isoforms.
• KEYWORDS from UniProt highlight roles in transport, ATP-binding, and membrane localization, along with proteomics identification.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight of ABCA6 is approximately 184 kDa. Ensure adequate protein transfer and use an appropriate gel percentage.
• As the antibody is polyclonal and raised against a specific peptide (amino acids 1518-1617), it may recognize full-length ABCA6 and possibly isoforms. Validate in your sample system.
• For ELISA, consider using the peptide immunogen as a coating antigen or perform sandwich ELISA with paired antibodies if available.
• Since ABCA6 is expressed in liver and macrophage-related tissues, using relevant cell lysates or tissue extracts may enhance detection.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop source for IVD raw materials, CamelBio connects diagnostic manufacturers and researchers with validated antibodies like this anti-ABCA6 rabbit polyclonal antibody for studying lipid transport pathways. Beyond this product, we supply antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents to support your assay development from concept to clinic. Contact us for bulk orders or custom sourcing of hard-to-find targets.