KCNU1 Rabbit Polyclonal Antibody for WB and ELISA - A8MYU2

Core Product Specifications and Parameters

Biological Background: KCNU1 Function and Localization

• KCNU1 (Potassium channel subfamily U member 1), also known as Slo3, KCa5, is a testis-specific calcium-activated potassium channel encoded by the KCNU1 gene (KCNMA3, KCNMC1, SLO3).
• It is activated by both intracellular pH and membrane voltage, mediating potassium efflux and representing the primary spermatozoan K+ current (PubMed:23129643, PubMed:24670955, PubMed:36649421). Related references: PMID:23129643 PMID:24670955 PMID:36649421
• KCNU1 underlies the pH-triggered membrane hyperpolarization critical for sperm capacitation as sperm encounter the alkaline environment of the female reproductive tract, and it is essential for male fertility (PubMed:34980136, PubMed:35551387, PubMed:36649421). Related references: PMID:34980136 PMID:35551387 PMID:36649421
• It localizes to the cell membrane and is enriched in the flagellum membrane of sperm.
• Mutations in KCNU1 are associated with spermatogenic failure (SPGF79), highlighting its role in reproductive health.
• Predicted to contain multiple transmembrane helices and a voltage-gated potassium channel domain.
• Exclusively expressed in the testis, consistent with its specialized reproductive function.

Experimental Guidance and Technical Tips

• For western blot analysis, a band around 130 kDa is expected; testis or sperm lysates may serve as positive controls.
• In ELISA applications, consider using recombinant KCNU1 protein or peptide as the coating antigen to capture the antibody.
• The antibody shows cross-reactivity with rat, suggesting conserved epitopes; validate performance in rat testis samples if required.
• As a polyclonal antibody, it may provide strong signal but could also produce background; optimize blocking and washing conditions.
• The immunogen corresponds to a C-terminal region (aa 920-1149); verify detection of full-length protein in native samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a one-stop sourcing solution for IVD raw materials, including specialized antibodies like this anti-KCNU1 polyclonal antibody for potassium channel and fertility research. We supply validated antibody pairs, optimized polyclonal/monoclonal antibodies, bulk ancillary reagents, and assist with rare target sourcing to accelerate your assay development from concept to clinic. Contact us to explore how our integrated procurement and technical support can streamline your project.