KCNK9 Polyclonal Antibody (Rabbit) for WB, ELISA - Q9NPC2

Core Product Specifications and Parameters

Biological Background: KCNK9 Function and Localization

• KCNK9 (TASK-3) is a voltage-dependent outward rectifying potassium channel that opens upon outward K+ flux, a gating mode essential for repolarization and setting resting membrane potential.
  Related references: PMID:11042359 PMID:11431495 PMID:26919430
• Extracellular acidification shifts the channel's ion selectivity from K+ to Na+ via protonation of the pH sensor His-98, driving a C-type inactivated state crucial for pH-dependent signaling.
  Related references: PMID:22948150 PMID:38630723
• The protein forms functional homo- and heterodimers (e.g., with KCNK3 or KCNK1), creating channels with distinct regulatory and gating properties.
  Related references: PMID:23169818 PMID:38630723
• In neurons, KCNK9 hyperpolarizes the resting membrane potential to limit basal excitability yet supports high-frequency action potential firing once threshold is crossed, contributing to fast-spiking behavior.
• Tissue expression is prominent in the cerebellum, hippocampus, retina, adrenal gland, kidney, and lung, where it mediates pH-sensitive currents and regulates hormone secretion (e.g., aldosterone).
• Subcellular localization includes the plasma membrane, mitochondrial inner membrane, and dendritic projections, reflecting diverse functional roles.
• Mutations in KCNK9 are associated with intellectual disability (BIBARS syndrome), underscoring its importance in neurodevelopment.
• Additional structural features include multiple transmembrane helices, N-glycosylation, and metal-binding sites, characteristics of the two-pore domain potassium channel family.

Experimental Guidance and Technical Tips

• For Western blot detection of endogenous KCNK9, expect a band near the predicted molecular weight of 42 kDa. Validate reactivity in lysates from human, mouse, or rat cerebellum or adrenal gland—tissues with known expression.
• The immunogen corresponds to an N-terminal peptide (aa 1-100); consider peptide blocking or competition assays to confirm band specificity.
• For ELISA applications, titrate the antibody to determine optimal working concentration and ensure linear detection of the target peptide or recombinant protein.
• Always include appropriate positive and negative controls, such as KCNK9-overexpressing cell lines and knockout samples, to validate antibody performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop-source for IVD raw materials, offering validated antibodies, recombinant proteins, and ancillary reagents to streamline your diagnostic and research workflows. This anti-KCNK9 antibody supports neuroscience and ion channel-focused projects, and our team can assist with custom bulk orders, matched antibody pair development, and technical consultation. From target identification to clinical assay design, CamelBio bridges the gap between concept and clinic with reliable supply and expert support.