KCNJ3 Rabbit pAb for WB, ELISA - P48549

Core Product Specifications and Parameters

Biological Background: KCNJ3 (GIRK1) Function and Localization

• G protein-activated inward rectifier potassium channel 1 (GIRK1, Kir3.1), encoded by the KCNJ3 gene, is a member of the inwardly rectifying potassium channel family.
• Functions as an inward rectifier potassium channel that allows potassium influx more readily than efflux; voltage dependence is modulated by extracellular potassium concentration and internal magnesium blockage (PubMed:8804710, PubMed:8868049). Related references: PMID:8804710 PMID:8868049
• Controlled by G proteins, this channel plays a crucial role in regulating the heartbeat (By similarity).
• Alternative splicing produces multiple isoforms, and the protein undergoes post-translational modifications including glycosylation and phosphorylation.
• Subcellular location is the membrane, where it contributes to potassium transport and voltage-gated channel activity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 50-150 of human KCNJ3; this region may be conserved across species, consistent with observed cross-reactivity with mouse and rat.
• For Western blot (WB), the predicted molecular weight is ~57 kDa. Consider using appropriate loading controls and optimizing membrane blocking conditions for detection in relevant sample lysates.
• For ELISA, validate the antibody with the target antigen and appropriate controls in your assay system.

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CamelBio streamlines IVD raw-material procurement, offering validated antibody pairs and optimized polyclonal antibodies like this anti-KCNJ3 antibody for ion channel and cardiac research. We also supply bulk ancillary reagents and assist with sourcing rare-target raw materials, supporting your diagnostic development from concept to clinic.