KBTBD7 Rabbit pAb - Q8WVZ9

Core Product Specifications and Parameters

Biological Background: KBTBD7 Function and Localization

• KBTBD7 encodes Kelch repeat and BTB domain-containing protein 7, a substrate adapter of the CUL3-RING E3 ubiquitin ligase complex (PubMed:25684205). Related references: PMID:25684205
• Functions in the CUL3(KBTBD6/7) complex to mediate ubiquitination and proteasomal degradation of the RAC1 guanine exchange factor TIAM1 via Lys-48-linked chains (PubMed:25684205). Related references: PMID:25684205
• Regulates RAC1 signal transduction and downstream processes, including cytoskeleton organization, cell migration, and cell proliferation (PubMed:25684205). Related references: PMID:25684205
• The ubiquitination of TIAM1 requires the membrane-associated protein GABARAP, which may locally restrict the activity of the ubiquitin ligase complex (PubMed:25684205). Related references: PMID:25684205
• Localizes to both the cytoplasm and nucleus (UniProt).
• Contains Kelch repeat and BTB domains, characteristic of substrate adaptors in CUL3-based ubiquitin ligases; classified as a signal transduction inhibitor (UniProt).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 475-684) of human KBTBD7; consider verifying target specificity using appropriate controls.
• For Western blot, a band at approximately 77 kDa is expected based on the predicted molecular weight; validate with relevant cell lysates.
• Cross-reactivity with mouse and rat orthologs is indicated; sequence alignment may guide expected reactivity in these species.
• ELISA applications may benefit from optimizing coating conditions and antibody titration for the specific assay format.
• Due to its role in ubiquitination, assess protein stability or degradation pathways in functional studies.

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