JNK3 Rabbit Monoclonal Antibody for WB and ELISA - P53779

Core Product Specifications and Parameters

Biological Background: JNK3 Function and Localization

• JNK3, also known as mitogen-activated protein kinase 10 (MAPK10), stress-activated protein kinase 1b (SAPK1b), and c-Jun N-terminal kinase 3, is a serine/threonine-protein kinase encoded by the MAPK10 gene (Gene ID: 5602). It belongs to the MAP kinase family and is specifically activated by stress stimuli.
• The protein plays a central role in the SAPK/JNK signaling pathway, where it is activated by upstream dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 in response to pro-inflammatory cytokines and physical stress. It phosphorylates transcription factors such as JUN, ATF2, JUND, and CLOCK-BMAL1, thereby regulating AP-1 transcriptional activity and circadian rhythms. Related references: PMID:22441692, PMID:22327296
• JNK3 is primarily involved in neuronal proliferation, differentiation, migration, and programmed cell death. It phosphorylates APP to regulate amyloid-beta precursor protein signaling during neuronal differentiation and modulates neurite growth in spiral ganglion neurons.
• The protein localizes to multiple cellular compartments including cytoplasm, membrane, nucleus, and mitochondrion, enabling diverse signaling roles.
• JNK3 shows tissue-specific expression restricted to a subset of neurons in the nervous system, with presence in hippocampus, cerebellum, striatum, brain stem, and weak expression in spinal cord. Very low expression in testis and kidney.
• Key post-translational modifications include phosphorylation (activation requires phosphorylation by MAP2K4/MAP2K7), and the protein is palmitoylated, which may affect membrane association. It also contains ATP-binding and nucleotide-binding domains essential for kinase activity.
• Additional keywords associated with JNK3 include biological rhythms, chromosomal rearrangement, epilepsy, and intellectual disability, reflecting its role in neurological processes and disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 350–450 of human MAPK10, a region that may be conserved across species given the reported cross-reactivity with mouse and rat. This could support multi-species studies.
• For WB, the expected molecular weight is approximately 44 kDa under reducing conditions. It is advisable to include appropriate positive controls such as neuronal cell lines (e.g., SH-SY5Y) or tissues known to express JNK3, and consider activating JNK3 with stress stimuli (e.g., anisomycin or UV) to enhance detection of the activated/phosphorylated form.
• For ELISA, the antibody may be used as a capture or detection reagent; optimization of coating concentration and blocking conditions is recommended. The synthetic peptide immunogen can serve as a positive control antigen.
• Since JNK3 expression is neuron-specific, tissue samples from brain regions (hippocampus, cerebellum) are more likely to yield positive signals compared to non-neuronal tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one‑stop supplier of IVD raw materials, supporting diagnostic developers with validated antibodies, recombinant proteins, and ancillary reagents from concept to clinic. This anti‑JNK3 rabbit monoclonal antibody is part of our stress signaling and neuroscience portfolio, ideal for apoptosis and circadian rhythm research. Contact us for bulk orders, custom conjugations, or help identifying complementary raw materials such as ELISA kits and peptide antigens.