JAK2 Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - O60674

Core Product Specifications and Parameters

Biological Background: JAK2 Function and Localization

• JAK2 (Janus kinase 2) is a non-receptor tyrosine-protein kinase that plays a central role in cytokine and growth factor signaling. It phosphorylates type I cytokine receptors (e.g., GHR, PRLR, EPOR, MPL) and type II receptors (e.g., IFN receptors, interleukin receptors), initiating downstream cascades (PubMed:15690087, PubMed:15899890, PubMed:7615558). Related references: PMID:15690087 PMID:15899890 PMID:7615558
• Upon receptor activation, JAK2 phosphorylates STAT proteins, leading to their dimerization, nuclear translocation, and activation of gene transcription. This mechanism is critical for processes like erythropoiesis, where EPO-induced JAK2 activation recruits and phosphorylates STAT5, which then upregulates erythropoiesis-related genes (PubMed:9618263, PubMed:9657743). Related references: PMID:9618263 PMID:9657743
• Beyond cytoplasmic signaling, JAK2 functions in the nucleus by phosphorylating histone H3 at tyrosine 41 (H3Y41ph). This modification excludes the heterochromatin protein CBX5 (HP1α) from chromatin, highlighting a direct role in chromatin regulation and epigenetic control (PubMed:19783980). Related references: PMID:19783980
• JAK2 is implicated in diverse signaling pathways, including angiotensin II-induced ARHGEF1 phosphorylation, CDKN1B phosphorylation to regulate cell cycle, and reciprocal phosphorylation with TEC to activate FOS transcription. It also modulates potassium channel activity of KCNA3 and participates in CNTF/OSM receptor signaling (PubMed:20098430, PubMed:21423214, PubMed:25644777, PubMed:11294841, PubMed:9188471). Related references: PMID:20098430 PMID:21423214 PMID:25644777 PMID:11294841 PMID:9188471
• Subcellularly, JAK2 is found in the endomembrane system, cytoplasm, and nucleus, enabling its multifaceted roles in signal transduction from membrane-proximal events to direct chromatin interactions.
• JAK2 is ubiquitously expressed across most tissues, consistent with its fundamental role in cytokine signaling and hematopoietic regulation. It is classified as a proto-oncogene, with chromosomal rearrangements and mutations linked to myeloproliferative diseases.

Experimental Guidance and Technical Tips

• The immunogen sequence (a.a. 933–1132) resides in the kinase domain of JAK2, which is well-conserved across human, mouse, and rat, supporting its predicted cross-reactivity. However, verify reactivity in your specific model system.
• For Western blotting (WB), the predicted band is ~131 kDa. Use appropriate loading controls and consider the use of phosphatase inhibitors to preserve phosphorylation states.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), JAK2 may show cytoplasmic and nuclear localization. Include appropriate negative and positive control tissues (e.g., bone marrow or spleen for hematopoietic expression).
• The antibody is suitable for ELISA; ensure optimal coating antigen and antibody dilution are determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, contract research organizations, and academic laboratories with integrated access to high-quality IVD raw materials. For JAK2 research—spanning hematological malignancies, cytokine signaling, and epigenetics—CamelBio can supply validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing services to accelerate your assay development and biomarker validation workflows. Our team bridges the gap from concept to clinic, ensuring reliable, scalable supply for your diagnostic needs.