JAB1/CSN5/COPS5 Rabbit Polyclonal Antibody for WB and ELISA - Q92905

Core Product Specifications and Parameters

Biological Background: JAB1/CSN5/COPS5 Function and Localization

• JAB1/CSN5/COPS5 is the catalytic subunit of the COP9 signalosome (CSN) complex, encoded by the COPS5 (CSN5, JAB1) gene, and also known as Jun activation domain-binding protein 1.
• The CSN complex is a central regulator of the ubiquitin-proteasome system, mediating the deneddylation of cullin subunits in SCF-type E3 ligases, which reduces their ubiquitin ligase activity.
• COPS5 serves as the probable catalytic center for cleavage of the ubiquitin-like protein Nedd8 from cullins, but requires other CSN subunits for full metalloprotease function.
• It interacts directly with a wide array of protein substrates, including p53/TP53, c-Jun/JUN, and BRSK2, and promotes their proteasomal degradation.
• CSN-dependent phosphorylation of TP53 and JUN, possibly via associated CK2 and PKD kinases, integrates phosphorylation with ubiquitination: promoting degradation of TP53 while protecting JUN from degradation.
• The protein dynamically localizes to multiple subcellular compartments—cytosol, nucleus, perinuclear region, and synaptic vesicles—reflecting its involvement in diverse signaling pathways.
• Post-translational modifications include acetylation, and the protein binds zinc, consistent with its metalloprotease activity.

Experimental Guidance and Technical Tips

• The immunogen covers full-length human JAB1/CSN5/COPS5 (amino acids 1–334), making this polyclonal antibody suitable for detecting the endogenous protein across human, mouse, and rat samples.
• For Western blotting, a band at approximately 38 kDa is expected; consider using whole-cell lysates and validate specificity with siRNA knockdown or KO controls.
• In ELISA applications, the antibody can be used as either capture or detection reagent; optimization of coating concentration and blocking conditions is recommended.
• Given the rabbit polyclonal nature, it may recognize both native and denatured conformations; testing in your specific sample matrix is advised.
• Consider appropriate secondary antibodies for your detection system, and note that cross-reactivity with other species has not been evaluated beyond human, mouse, and rat.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material procurement, providing ready access to validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing. This JAB1/CSN5/COPS5 antibody supports research into ubiquitin conjugation, COP9 signalosome biology, and proteasomal degradation pathways. Whether you are scaling up assay development or sourcing niche raw materials, CamelBio offers the supply chain and technical support to accelerate your project from concept to clinic.