ITPK1 Rabbit pAb - Q13572

Core Product Specifications and Parameters

Biological Background: ITPK1 Function and Localization

• ITPK1 is a human kinase/phosphatase/isomerase belonging to the inositol phosphate metabolism pathway, with alternative splice variants reported.
• ITPK1 phosphorylates Ins(3,4,5,6)P4 at position 1 and Ins(1,3,4)P3 at positions 5 and 6, playing a key role in inositol phosphate synthesis. Related references: PMID:11042108 PMID:8662638
• The protein also acts as an inositol polyphosphate phosphatase, dephosphorylating Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3. Related references: PMID:11909533 PMID:17616525
• ITPK1 may interconvert Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Related references: PMID:11909533
• It modulates TNF-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain. Related references: PMID:11909533 PMID:12925536 PMID:17616525
• ITPK1 is essential for MLKL-mediated necroptosis by producing highly phosphorylated inositol phosphates that activate MLKL, leading to membrane localization. Related references: PMID:17616525
• ITPK1 is expressed in multiple tissues, with highest levels in brain, and is also found in heart, skeletal muscle, kidney, pancreas, liver, placenta, and lung.
• The protein is subject to acetylation and phosphorylation and requires magnesium as a cofactor.

Experimental Guidance and Technical Tips

• The immunogen (recombinant fusion protein corresponding to amino acids 185-414) covers the C-terminal region of human ITPK1; this region may confer target specificity.
• For Western blotting, use appropriate positive control lysates from human, mouse, or rat tissues expressing ITPK1 (e.g., brain or heart) and optimize antibody dilution.
• For ELISA, coat with immunogen or target protein and validate under standard assay conditions.
• Note that the predicted molecular weight is ~46 kDa, but actual migration may vary depending on post-translational modifications and gel conditions.

CamelBio: Your One-Stop Sourcing Bridge

As a committed supplier of IVD raw materials, CamelBio provides validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents to support research and diagnostic development. This ITPK1 antibody is suitable for inositol phosphate signaling studies, apoptosis, and necroptosis research. For bulk orders, custom formulations, or technical consultation, CamelBio offers end-to-end support from concept to clinic.