ITGB3 Rabbit Polyclonal Antibody for WB, FC, ELISA - P05106

Core Product Specifications and Parameters

Biological Background: ITGB3 Function and Localization

• Integrin beta-3 (ITGB3), also known as GPIIIa or CD61, is the beta subunit that heterodimerizes with alpha-V or alpha-IIb integrins. Two major isoforms exist: beta-3A is widely expressed and enriched in osteoblasts, while beta-3C is specific to prostate and testis.
• Integrin alpha-IIb/beta-3 (GPIIb/IIIa) mediates platelet aggregation through fibrinogen binding, physically plugging ruptured endothelial surfaces—a critical hemostatic event. Related references: PMID:9111081
• ITGAV:ITGB3 serves as a receptor for a broad array of RGD-containing ligands (e.g., vitronectin, fibronectin, osteopontin) and also acts as a coreceptor for growth factors/cytokines, including NRG1, FGF1, FGF2, IGF1, IGF2, and IL1B, thereby playing essential roles in their signaling pathways. Related references: PMID:20682778, PMID:18441324, PMID:28302677, PMID:19578119, PMID:28873464, PMID:29030430
• ITGAV:ITGB3 also interacts with PLA2G2A via a distinct site, inducing conformational changes that enhance ligand binding. Additionally, it functions as a receptor for fibrillin-1, CD40LG, IBSP, and the erythrocyte ICAM4 ligand, mediating cell adhesion and migration. Related references: PMID:18635536, PMID:25398877, PMID:12807887, PMID:31331973, PMID:10640428, PMID:12477717
• Subcellularly, ITGB3 localizes to the cell membrane, focal adhesions, lamellipodia, and cell junctions. In neurons, it is present at postsynaptic membranes and synapses, where it regulates serotonin reuptake and AMPA receptor endocytosis, influencing synaptic plasticity.
• The protein harbors EGF-like domains, disulfide bonds, and metal-binding sites that coordinate calcium and magnesium. Post‑translational modifications include phosphorylation and N‑linked glycosylation, which affect its molecular weight and function.
• Notably, ITGAV:ITGB3 acts as a host receptor for multiple viruses, including herpesvirus 8, coxsackievirus A9, hantaan virus, and HIV‑1 (via Tat protein), implicating it in pathogen entry.

Experimental Guidance and Technical Tips

• The immunogen (aa 21–718) covers nearly the entire extracellular domain; the resulting polyclonal antibody is expected to recognize both native and denatured ITGB3 across human, mouse, and rat samples.
• For Western blotting, a band around 86–87 kDa is anticipated under reducing conditions, though glycosylation may cause slight upward shifts. Validate specificity using appropriate positive and negative controls.
• In flow cytometry, test the antibody under fixation/permeabilization conditions relevant to your cell type, as surface expression may require metabolic activity or activation (e.g., platelet agonists).
• For ELISA, the antibody can be explored as a capture or detection reagent, but optimal pairing and coating conditions should be determined empirically.
• Consider cross‑species reactivity carefully when moving between human, mouse, and rat models; sequence alignment suggests conserved epitopes but localized differences may affect binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw‑material partner, offering diagnostic manufacturers and research labs one‑stop access to high‑quality antibodies like anti‑ITGB3. Our portfolio includes validated antibody pairs, optimized polyclonals, and bulk ancillary reagents, supporting projects in platelet biology, thrombosis diagnostics, and infection research. With end‑to‑end technical consultation from concept to clinic, CamelBio streamlines your sourcing of even hard‑to‑find targets, ensuring robust supply for development and production.