ISG15 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P05161

Core Product Specifications and Parameters

Biological Background: ISG15 Function and Localization

• ISG15 is a ubiquitin-like protein that serves a crucial role in the innate immune response to viral infection, acting either via ISGylation of target proteins or as an unconjugated secreted signaling molecule. Related references: PMID:27564865 PMID:39465252
• ISGylation involves a cascade of E1, E2, and E3 enzymes that conjugate ISG15 to lysine residues on target proteins including IFIT1, MX1, IFIH1/MDA5, and IRF3, thereby modulating antiviral signaling. Related references: PMID:33727702
• The protein exhibits antiviral activity against both DNA and RNA viruses; it disrupts HIV-1 and Ebola virus budding and ISGylates influenza A virus NS1 protein to inhibit replication.
• Secreted ISG15 binds the ITGAL/ITGB2 integrin receptor to activate SRC family kinases, leading to secretion of IFNG and IL10, and plays an essential role in antimycobacterial immunity. Related references: PMID:29100055
• Subcellularly, ISG15 is localized in the cytoplasm and is also secreted into the extracellular space.
• Tissue expression is detected in lymphoid cells, striated and smooth muscle, epithelia, and neurons; overexpression correlates with advanced stages in pancreatic, endometrial, and bladder cancers.
• Keywords associated with ISG15 include Antiviral defense, Cytoplasm, Secreted, Innate immunity, and Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human ISG15, so the polyclonal antibody may recognize multiple epitopes; consider using it in both native and denaturing conditions.
• For WB, use interferon-stimulated cell lysates (e.g., IFN-α/β treated HeLa or A549) as positive controls to induce ISG15 expression.
• For IHC-P, validate staining patterns with known tissue controls; ISG15 upregulation in certain cancers may serve as a relevant model.
• In ELISA, titrate the antibody with recombinant ISG15 protein to establish optimal working concentrations.
• Given cross-reactivity with rat, verify expected bands in rat samples if included in experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a comprehensive one-stop platform for IVD raw materials, from concept to clinic. For ISG15 and innate immunity research, we provide validated antibody pairs, optimized polyclonal antibodies like this ISG15 rabbit pAb, bulk ancillary reagents, and rare target raw-material sourcing to streamline your diagnostic development.